Unique chromosomal regions associated with virulence of an avian pathogenic Escherichia coli strain

The avian pathogenic Escherichia coli strain (chi)7122 (serotype O78:K80:H9) causes airsacculitis and colisepticemia in chickens, To identify genes associated with avian disease, a genomic subtraction technique was performed between strain (chi)7122 and the E. coli K-12 strain (chi)289, The DNA isolated using this method was found only in strain (chi)7122 and was used to identify cosmid clones carrying unique DNA from a library of (chi)7122 that were then used to map the position of unique DNA on the E. coli chromosome, A total of 12 unique regions were found, 5 of which correspond to previously identified positions for unique DNA sequence in E. coli strains, To assess the role each unique region plays in virulence, mutants of (chi)7122 were constructed in which a segment of unique DNA was replaced with E. coli K-12 DNA by cotransduction of linked transposon insertions in DNA flanking the unique sequence, The resulting replacement mutants were assessed for inability to colonize the air sac and cause septicemia in 2-week-old white Leghorn chickens, Two mutants were found to be avirulent when injected into the right caudal air sac of 2-week-old chickens, One avirulent mutant, designated (chi)7145, carries a replacement of the rfb locus at 44 min, generating a rough phenotype, The second mutant is designated (chi)7146, and carries a replacement at position 0.0 min on the genetic map, Both mutants could be complemented to partial virulence by cosmids carrying sequences unique to (chi)7122.