Substitution at codon 269 (glutamine->proline) of the leptin receptor (OB-R) cDNA is the only mutation found in the Zucker fatty (fa/fa) rat

被引:160
作者
Iida, M
Murakami, T
Ishida, K
Mizuno, A
Kuwajima, M
Shima, K
机构
[1] UNIV TOKUSHIMA,SCH MED,DEPT LAB MED,TOKUSHIMA 770,JAPAN
[2] OTSUKA PHARMACEUT CO LTD,OTSUKA ASSAY LABS,TOKUSHIMA 77101,JAPAN
关键词
D O I
10.1006/bbrc.1996.1070
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We recently cloned one of spliced variant forms of rat leptin receptor (OB-R), which contains a short intracellular domain, and found obese-phenotype-linked nucleotide alteration in the extracellular domain of the cDNA from the Zucker (fa/fa) rat, which results in a glutamine(269) to proline(269) amino acid substitution. Reported herein are the cloning and sequencing of another spliced variant forms of rat OB-R cDNA with a long intracellular domain. Both forms of OB-R cDNA share the same extracellular domain. In the Zucker (fa/fa) rat, no changes in either the gene structure nor in the nucleotide sequence of the long intracellular domain were observed. However, the expression level of OB-R mRNA in the brain of Zucker (fa/fa) rat was higher than for lean littermates. These facts suggest that the substitution at codon 269 of the OB-R cDNA represents the crucial mutation which results in the obese phenotype of Zucker (fa/fa) rat. (C) 1996 Academic Press, Inc.
引用
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页码:597 / 604
页数:8
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