Genetic engineering of the chloroplast: novel tools and new applications

被引：85

作者：

Bock, Ralph ^{[1
]}

机构：

[1] Max Planck Inst Mol Pflanzenphysiol, D-14476 Potsdam, Germany

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2014年 / 26卷

基金：

欧盟第七框架计划;

关键词：

PLASTID TRANSFORMATION; TRANSPLASTOMIC TOBACCO; TRANSGENE EXPRESSION; FOREIGN PROTEIN; ACCUMULATION; LEVEL; IDENTIFICATION; GENOME; PLANTS; FUSION;

D O I：

10.1016/j.copbio.2013.06.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The plastid genome represents an attractive target of genetic engineering in crop plants. Plastid transgenes often give high expression levels, can be stacked in operons and are largely excluded from pollen transmission. Recent research has greatly expanded our toolbox for plastid genome engineering and many new proof-of-principle applications have highlighted the enormous potential of the transplastomic technology in both crop improvement and the development of plants as bioreactors for the sustainable and cost-effective production of biopharmaceuticals, enzymes and raw materials for the chemical industry. This review describes recent technological advances with plastid transformation in seed plants. It focuses on novel tools for plastid genome engineering and transgene expression and summarizes progress with harnessing the potential of plastid transformation in biotechnology.

引用

页码：7 / 13

页数：7

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