Characterization of water-soluble organic matter in soils by size exclusion chromatography and fractionation with polyvinylpyrrolidone

被引:5
作者
Aoyama, M [1 ]
机构
[1] Hirosaki Univ, Fac Agr & Life Sci, Hirosaki, Aomori 0368561, Japan
关键词
fulvic acid; humic acid; polyvinylpyrrolidone; size exclusion chromatography; water-soluble organic matter;
D O I
10.1080/00380768.2002.10409229
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Water extracts were obtained from four types of soils (Brown Lowland soil, Yellow soil with manure application for 6 years, non-allophanic Andosol, and allophanic Andosol), and the organic matter in the water extracts was fractionated according to the solubility in acid and adsorption onto polyvinylpyrrolidone (PVP). For the water extracts and their fractions, the amounts of organic C, total N, and anthrone-reactive C (ARC) were analyzed, and high performance size exclusion chromatography (HPSEC) was carried out. The PVP-non-adsorbed fulvic acid (FA) fraction accounted for the largest proportion of the total water-soluble organic C, ranging from 52% for the Yellow soil to 96% for the allophanic Amdosol, followed by the PVP-adsorbed FA and humic acid (RA) fractions. The water extract of the allophanic Andosol was characterized by the lack of HA fraction and a small proportion of PVP-adsorbed FA fraction. For all the water extract samples, more than 90% of the ARC was recovered in the PVP-non-adsorbed FA fraction. The proportion of ARC in the total organic C in the fraction was also highest in the PVP-non-adsorbed FA fraction. The molecular weight (MW) of the humic substances (HS) at peak maximum was estimated at 1,300 Da for the water extracts and their fractions from the Brown Lowland soil and non-allophanic Andosol samples by HPSEC using polyethylene glycols as MW standards. Manure application increased the MW of HS in the HA and PVP-adsorbed FA fractions. On the other hand, only a small amount of HS was found in the water extract of the allophanic Andosol by HPSEC.
引用
收藏
页码:475 / 481
页数:7
相关论文
共 32 条
[1]   Factors affecting microbial biomass and dehydrogenase activity in apple orchard soils with heavy metal accumulation [J].
Aoyama, M ;
Nagumo, T .
SOIL SCIENCE AND PLANT NUTRITION, 1996, 42 (04) :821-831
[4]   Characterization of groundwater humic substances: influence of sedimentary organic carbon [J].
Artinger, R ;
Buckau, G ;
Geyer, S ;
Fritz, P ;
Wolf, M ;
Kim, JI .
APPLIED GEOCHEMISTRY, 2000, 15 (01) :97-116
[5]   DETERMINATION OF MOLECULAR-WEIGHT DISTRIBUTIONS OF FULVIC AND HUMIC ACIDS USING FLOW FIELD-FLOW FRACTIONATION [J].
BECKETT, R ;
JUE, Z ;
GIDDINGS, JC .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1987, 21 (03) :289-295
[6]   GEL-FILTRATION CHROMATOGRAPHY OF HUMIC SUBSTANCES IN SOIL SOLUTIONS USING HPLC-DETERMINATION OF THE MOLECULAR-WEIGHT DISTRIBUTION [J].
BERDEN, M ;
BERGGREN, D .
JOURNAL OF SOIL SCIENCE, 1990, 41 (01) :61-72
[7]   Bioavailability of water extractable organic carbon fractions in forest and agricultural soil profiles [J].
Boyer, JN ;
Groffman, PM .
SOIL BIOLOGY & BIOCHEMISTRY, 1996, 28 (06) :783-790
[8]   RELATIONSHIPS BETWEEN DENITRIFICATION CAPACITIES OF SOILS AND TOTAL, WATER-SOLUBLE AND READILY DECOMPOSABLE SOIL ORGANIC-MATTER [J].
BURFORD, JR ;
BREMNER, JM .
SOIL BIOLOGY & BIOCHEMISTRY, 1975, 7 (06) :389-394
[9]   DISSOLVED ORGANIC-CARBON IN OLD FIELD SOILS - TOTAL AMOUNTS AS A MEASURE OF AVAILABLE RESOURCES FOR SOIL MINERALIZATION [J].
COOK, BD ;
ALLAN, DL .
SOIL BIOLOGY & BIOCHEMISTRY, 1992, 24 (06) :585-594
[10]   DISSOLVED ORGANIC-CARBON IN OLD FIELD SOILS - COMPOSITIONAL CHANGES DURING THE BIODEGRADATION OF SOIL ORGANIC-MATTER [J].
COOK, BD ;
ALLAN, DL .
SOIL BIOLOGY & BIOCHEMISTRY, 1992, 24 (06) :595-600