Contractile properties of in situ perfused skeletal muscles from rats with congestive heart failure

被引:28
作者
Lunde, PK [1 ]
Verburg, E [1 ]
Eriksen, M [1 ]
Sejersted, OM [1 ]
机构
[1] Univ Oslo, Ullevaal Hosp, Expt Med Res Inst, N-0407 Oslo, Norway
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2002年 / 540卷 / 02期
关键词
D O I
10.1113/jphysiol.2001.013324
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We hypothesized that in congestive heart failure (CHF) slow-twitch but not fast-twitch muscles exhibit decreased fatigue resistance in the sense of accelerated reduction of muscle force during activity. Experiments were carried out on anaesthetized rats 6 weeks after induction of myocardial infarction or a sham operation (Sham). Animals with left ventricular end-diastolic pressure (LVEDP) > 15 mmHg under anaesthesia were selected for the CHF group. There was no muscle atrophy in CHF. Force generation by in situ perfused soleus (Sol) or extensor digitorum longus (EDL) muscles was recorded during stimulation (trains at 5 Hz for 6 s (Sol) or 10 Hz for 1.5 s (EDL) at 10 or 2.5 s intervals, respectively) for I h in Sol and 10 min in EDL at 37degreesC. Initial force was almost the same in Sol from CHF and Sham rats, but relaxation was slower in CHF. Relaxation times (95-5% of peak force) were 177 +/- 55 and 131 +/- 44 ms in CHF and Sham, respectively, following the first stimulation train. After 2 min of stimulation the muscles transiently became slower and maximum relaxation times were 264 +/- 71 and 220 +/- 45 ms in CHF and Sham, respectively (P < 0.05). After 60 min they recovered to 204 +/- 60 and 122 +/- 55 ms in CHF and Sham, respectively (P < 0.05). In CHF but not in Sham rats the force of contraction of Sol declined from the second to the sixtieth minute to 70% of peak force. The EDL of both CHF and Sham fatigued to 24-28% of initial force, but no differences in contractility pattern were detected. Thus, slow-twitch muscle is severely affected in CHF by slower than normal relaxation and significantly reduced fatigue resistance, which may explain the sensation of both muscle stiffness and fatigue in CHF patients.
引用
收藏
页码:571 / 580
页数:10
相关论文
共 31 条
  • [1] Muscle unloading induces slow to fast transitions in myofibrillar but not mitochondrial properties. Relevance to skeletal muscle abnormalities in heart failure
    Bigard, AX
    Boehm, E
    Veksler, V
    Mateo, P
    Anflous, K
    Ventura-Clapier, R
    [J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1998, 30 (11) : 2391 - 2401
  • [2] 3 MYOSIN ADENOSINE TRIPHOSPHATASE SYSTEMS - NATURE OF THEIR PH LABILITY AND SULFHYDRYL DEPENDENCE
    BROOKE, MH
    KAISER, KK
    [J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1970, 18 (09) : 670 - &
  • [3] RAT SKELETAL-MUSCLE METABOLISM IN EXPERIMENTAL HEART-FAILURE - EFFECTS OF PHYSICAL-TRAINING
    BRUNOTTE, F
    THOMPSON, CH
    ADAMOPOULOS, S
    COATS, A
    UNITT, J
    LINDSAY, D
    KAKLAMANIS, L
    RADDA, GK
    RAJAGOPALAN, B
    [J]. ACTA PHYSIOLOGICA SCANDINAVICA, 1995, 154 (04): : 439 - 447
  • [4] MULTIVARIATE CLASSIFICATION OF HISTOCHEMICALLY STAINED HUMAN SKELETAL-MUSCLE FIBERS BY THE SIMCA METHOD
    BYE, E
    GRONNEROD, O
    VOGT, NB
    [J]. HISTOCHEMICAL JOURNAL, 1989, 21 (01): : 15 - 22
  • [5] INTRACELLULAR CALCIUM HOMEOSTASIS
    CARAFOLI, E
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 : 395 - 433
  • [6] SKELETAL-MUSCLE PHOSPHATE-METABOLISM ABNORMALITIES IN VOLUME-OVERLOAD EXPERIMENTAL HEART-FAILURE
    CHATI, Z
    ZANNAD, F
    MICHEL, C
    LHERBIER, B
    MERTES, PM
    ESCANYE, JM
    RIBUOT, C
    ROBERT, J
    VILLEMOT, JP
    ALIOT, E
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1994, 267 (06): : H2186 - H2192
  • [7] AFTER-EFFECTS OF REPETITIVE STIMULATION ON ISOMETRIC TWITCH CONTRACTION OF RAT FAST SKELETAL MUSCLE
    CLOSE, R
    HOH, JFY
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1968, 197 (02): : 461 - &
  • [8] INFLUENCE OF TEMPERATURE ON ISOMETRIC CONTRACTIONS OF RAT SKELETAL MUSCLES
    CLOSE, R
    HOH, JFY
    [J]. NATURE, 1968, 217 (5134) : 1179 - &
  • [9] Role of myoplasmic phosphate in contractile function of skeletal muscle: studies on creatine kinase-deficient mice
    Dahlstedt, AJ
    Katz, A
    Westerblad, H
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 2001, 533 (02): : 379 - 388
  • [10] Variation of phospholamban in slow-twitch muscle sarcoplasmic reticulum between mammalian species and a link to the substrate specificity of endogenous Ca2+-calmodulin-dependent protein kinase
    Damiani, E
    Sacchetto, R
    Margreth, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1464 (02): : 231 - 241