On-Line Monitoring Imidacloprid and Thiacloprid in Celery Juice Using Quartz Crystal Microbalance

In this article, we report a quartz crystal microbalance (QCM)-based detection method which allows the identification and quantification of two neonicotinoid pesticides, imidacloprid and thiacloprid, in aqueous solutions and celery juice. To achieve high selectivity, molecular imprinted monolayers (MIMs), which can either recognize 1 mu M of imidacloprid or 1 mu M of thiacloprid, are prepared from alkanethiols self-assembled on QCM sensor chips with preadsorbed templates (either imidacloprid or thiacloprid). Our experimental results show that the detection limit can be improved by using alkanethiols having longer hydrocarbon chains. For example, MIMs prepared from hexadecanethiol have dissociation constants 2-5 times smaller than those prepared from octanethiol. To detect two neonicotinoids in vegetable samples simultaneously, we also develop a new type of MIM with two different templates. A single QCM decorated with this MIM can respond to 10 mu M of imidacloprid and 10 mu M thiacloprid in celery juice in a real-time manner.