Mechanism-based profiling of enzyme families

The literature includes a number of studies expanding the technological scope and biological applications of activity-based protein profiling (ABPP). This review covers these advances and present their findings within the prevailing themes that have been served as the foundation for ABPP. Focus is on the explanation of ABP design to highlight how the structure and reactivity of these reagents influences their target portfolio and the scope of ABPP experiments. The review is presented into sections: (1) the design of ABPs for functional proteomics, (2) biological applications of ABPs, (3) methodological advances beyond the central paradigm and (4) ABPs and their application beyond comparative proteomics.