Extracellular vesicles from oral squamous carcinoma cells display pro- and anti-angiogenic properties

被引：16

作者：

de Andrade, A. L. D. L. ^{[1
,2
]}

de Oliveira, C. E. ^{[2
]}

Dourado, M. R. ^{[2
]}

Macedo, C. C. S. ^{[2
]}

Winck, F. V. ^{[3
]}

Paes Leme, A. F. ^{[3
]}

Salo, T. ^{[2
,4
,5
]}

Coletta, R. D. ^{[2
]}

de Almeida Freitas, R. ^{[1
]}

Galvao, H. C. ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Norte, Fac Dent, Natal, RN, Brazil

[2] Univ Estadual Campinas, Sch Dent, Dept Oral Diag, Piracicaba, Brazil

[3] CNPEM, LNBio, Biosci Natl Lab, Mass Spectrometry Lab, Campinas, SP, Brazil

[4] Univ Oulu, Oulu Univ Hosp, Med Res Ctr Oulu, Unit Canc Res & Translat Med,Fac Med, Oulu, Finland

[5] Univ Helsinki, Helsinki Univ Hosp, Inst Oral & Maxillofacial Dis, Dept Pathol,HUSLAB, Helsinki, Finland

来源：

ORAL DISEASES | 2018年 / 24卷 / 05期

基金：

巴西圣保罗研究基金会;

关键词：

angiogenesis; extracellular vesicles; oral cancer; TUMOR-DERIVED EXOSOMES; CANCER; MICROVESICLES;

D O I：

10.1111/odi.12765

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

BackgroundA new intercellular communication mode established by neoplastic cells and tumor microenvironment components is based on extracellular vesicles (EVs). However, the biological effects of the EVs released by tumor cells on angiogenesis are not completely understood. Here, we aimed to understand the biological effects of EVs isolated from two cell lines of oral squamous cell carcinoma (OSCC) (SCC15 and HSC3) on endothelial cell tubulogenesis. MethodsOSCC-derived EVs were isolated with a polymer-based precipitation method, quantified using nanoparticle tracking analysis and verified for EV markers by dot blot. Functional assays were performed to assess the angiogenic potential of the OSCC-derived EVs. ResultsThe results showed that EVs derived from both cell lines displayed typical spherical-shaped morphology and expressed the EV markers CD63 and Annexin II. Although the average particle concentration and size were quite similar, SCC15-derived EVs promoted a pronounced tubular formation associated with significant migration and apoptosis rates of the endothelial cells, whereas EVs derived from HSC3 cells inhibited significantly endothelial cell tubulogenesis and proliferation. ConclusionThe findings of this study reveal that EVs derived from different OSCC cell lines by a polymer-based precipitation method promote pro- or anti-angiogenic effects.

引用

页码：725 / 731

页数：7

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