Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides

gamma-Glycerylpropylsilyl or "glycopliase" glass has been promoted as a non-fouling surface, resistant to protein adsorption and cell attachment, oil which one can immobilize oligopeptide ligands, and thus create cell-type-specific culture surfaces. The present study confirmed that the glycerol-rich glycophase surface is useful for peptide immobilization. But glycophase glass was observed to adsorb more albumin than glass. At pH 7.4, desorption Studies revealed that albmin bound more glass than to glass. Moreover, the growth rates, morphologies, and functions of trangenic betaG 1/17 insulinoma cell cultures were equivalent on the two Surfaces. Glycophase glass is neither protein- nor cell-repellant. (C) 2002 Elsevier Science Ltd. All rights reserved.