Control of stochasticity in eukaryotic gene expression

被引:1095
作者
Raser, JM [1 ]
O'Shea, EK [1 ]
机构
[1] Univ Calif San Francisco, Dept Biochem & Biophys, Howard Hughes Med Inst, San Francisco, CA 94143 USA
关键词
D O I
10.1126/science.1098641
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Noise, or random fluctuations, in gene expression may produce variability in cellular behavior. To measure the noise intrinsic to eukaryotic gene expression, we quantified the differences in expression of two alleles in a diploid cell. We found that such noise is gene-specific and not dependent on the regulatory pathway or absolute rate of expression. We propose a model in which the balance between promoter activation and transcription influences the variability in messenger RNA levels. To confirm the predictions of our model, we identified both cis- and trans-acting mutations that alter the noise of gene expression. These mutations suggest that noise is an evolvable trait that can be optimized to balance fidelity and diversity in eukaryotic gene expression.
引用
收藏
页码:1811 / 1814
页数:4
相关论文
共 23 条
[1]   NUCLEASE HYPERSENSITIVE REGIONS WITH ADJACENT POSITIONED NUCLEOSOMES MARK THE GENE BOUNDARIES OF THE PHO5/PHO3 LOCUS IN YEAST [J].
ALMER, A ;
HORZ, W .
EMBO JOURNAL, 1986, 5 (10) :2681-2687
[2]  
Arkin A, 1998, GENETICS, V149, P1633
[3]   Multiple mechanistically distinct functions of SAGA at the PH05 promoter [J].
Barbaric, S ;
Reinke, H ;
Hörz, W .
MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (10) :3468-3476
[4]   Increasing the rate of chromatin remodeling and gene activation -: a novel role for the histone acetyltransferase Gcn5 [J].
Barbaric, S ;
Walker, J ;
Schmid, A ;
Svejstrup, JQ ;
Hörz, W .
EMBO JOURNAL, 2001, 20 (17) :4944-4951
[5]   Noise in eukaryotic gene expression [J].
Blake, WJ ;
Kærn, M ;
Cantor, CR ;
Collins, JJ .
NATURE, 2003, 422 (6932) :633-637
[6]   Nucleosomes unfold completely at a transcriptionally active promoter [J].
Boeger, H ;
Griesenbeck, J ;
Strattan, JS ;
Kornberg, RD .
MOLECULAR CELL, 2003, 11 (06) :1587-1598
[7]   The product of the SNF2/SWI2 paralogue INO80 of Saccharomyces cerevisiae required for efficient expression of various yeast structural genes is part of a high-molecular-weight protein complex [J].
Ebbert, R ;
Birkmann, A ;
Schüller, HJ .
MOLECULAR MICROBIOLOGY, 1999, 32 (04) :741-751
[8]   Stochastic gene expression in a single cell [J].
Elowitz, MB ;
Levine, AJ ;
Siggia, ED ;
Swain, PS .
SCIENCE, 2002, 297 (5584) :1183-1186
[9]   ROLE OF TRANS-ACTIVATING PROTEINS IN THE GENERATION OF ACTIVE CHROMATIN AT THE PHO5 PROMOTER IN SACCHAROMYCES-CEREVISIAE [J].
FASCHER, KD ;
SCHMITZ, J ;
HORZ, W .
EMBO JOURNAL, 1990, 9 (08) :2523-2528
[10]   STRUCTURAL AND FUNCTIONAL REQUIREMENTS FOR THE CHROMATIN TRANSITION AT THE PHO5 PROMOTER IN SACCHAROMYCES-CEREVISIAE UPON PHO5 ACTIVATION [J].
FASCHER, KD ;
SCHMITZ, J ;
HORZ, W .
JOURNAL OF MOLECULAR BIOLOGY, 1993, 231 (03) :658-667