Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR

被引：66

作者：

Cortez, A. L. L.

Carvalho, A. C. F. B.

Ikuno, A. A.

Burger, K. P.

Vidal-Martins, A. M. C.

机构：

[1] Univ Estadual Paulista, Fac Ciencias Agrarias & Vet Jaboticabal, Dept Med Vet Prevent & Reproducao Anim, BR-14884900 Jaboticabal, SP, Brazil

[2] Inst Biol, BR-04014002 Sao Paulo, Brazil

来源：

RESEARCH IN VETERINARY SCIENCE | 2006年 / 81卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

Salmonella spp; Salmonella Enteritidis; Salmonella Typhimurium; multiplex-PCR; chicken abattoir;

D O I：

10.1016/j.rvsc.2006.03.006

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 [兽医学];

摘要：

The present study was carried out to report the occurrence Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs. Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the inuA, pefA, and sefA gene sequences from Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：340 / 344

页数：5

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