Selective plane illumination microscopy techniques in developmental biology

被引:439
作者
Huisken, Jan [1 ]
Stainier, Didier Y. R.
机构
[1] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94158 USA
[2] Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94158 USA
来源
DEVELOPMENT | 2009年 / 136卷 / 12期
关键词
SHEET-BASED MICROSCOPY; HIGH-RESOLUTION; IMPROVES RESOLUTION; RECONSTRUCTION; SPECIMENS; DEEP; ULTRAMICROSCOPY; MIGRATION; SPIM;
D O I
10.1242/dev.022426
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.
引用
收藏
页码:1963 / 1975
页数:13
相关论文
共 45 条
[1]   Zebrafish model for human long QT syndrome [J].
Arnaout, Rima ;
Ferrer, Tania ;
Huisken, Jan ;
Spitzer, Kenneth ;
Stainier, Didier Y. R. ;
Tristani-Firouzi, Martin ;
Chi, Neil C. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (27) :11316-11321
[2]   Ultramicroscopy: 3D reconstruction of large microscopical specimens [J].
Becker, K. ;
Jaehrling, N. ;
Kramer, E. R. ;
Schnorrer, F. ;
Dodt, H. -U .
JOURNAL OF BIOPHOTONICS, 2008, 1 (01) :36-42
[3]   Lateral modulation boosts image quality in single plane illumination fluorescence microscopy [J].
Breuninger, Tobias ;
Greger, Klaus ;
Stelzer, Ernst H. K. .
OPTICS LETTERS, 2007, 32 (13) :1938-1940
[4]   Design and quantitative resolution measurements of an optical virtual sectioning three-dimensional imaging technique for biomedical specimens, featuring two-micrometer slicing resolution [J].
Buytaert, Jan A. N. ;
Dirckx, Joris J. J. .
JOURNAL OF BIOMEDICAL OPTICS, 2007, 12 (01)
[5]   Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure [J].
Denk, W ;
Horstmann, H .
PLOS BIOLOGY, 2004, 2 (11) :1900-1909
[6]   Ultramicroscopy:: three-dimensional visualization of neuronal networks in the whole mouse brain [J].
Dodt, Hans-Ulrich ;
Leischner, Ulrich ;
Schierloh, Anja ;
Jaehrling, Nina ;
Mauch, Christoph Peter ;
Deininger, Katrin ;
Deussing, Jan Michael ;
Eder, Matthias ;
Zieglgaensberger, Walter ;
Becker, Klaus .
NATURE METHODS, 2007, 4 (04) :331-336
[7]   Optically sectioned imaging by oblique plane microscopy [J].
Dunsby, C. .
OPTICS EXPRESS, 2008, 16 (25) :20306-20316
[8]   Three-dimensional laser microsurgery in light-sheet based microscopy (SPIM) [J].
Engelbrecht, Christoph J. ;
Greger, Klaus ;
Reynaud, Emmanuel G. ;
Krzic, Uros ;
Colombelli, Julien ;
Stelzer, Ernst H. K. .
OPTICS EXPRESS, 2007, 15 (10) :6420-6430
[9]   Resolution enhancement in a light-sheet-based microscope (SPIM) [J].
Engelbrecht, Christoph J. ;
Stelzer, Ernst H. K. .
OPTICS LETTERS, 2006, 31 (10) :1477-1479
[10]   Surface imaging microscopy, an automated method for visualizing whole embryo samples in three dimensions at high resolution [J].
Ewald, AJ ;
McBride, H ;
Reddington, M ;
Fraser, SE ;
Kerschmann, R .
DEVELOPMENTAL DYNAMICS, 2002, 225 (03) :369-375