Matrigel cytometry: A novel method for quantifying angiogenesis in vivo

被引:48
作者
Adini, A. [1 ]
Fainaru, O.
Udagawa, T.
Connor, K. M.
Folkman, J.
D'Amato, R. J.
机构
[1] Harvard Univ, Sch Med, Dept Surg, Childrens Hosp,Vasc Biol Program, Boston, MA 02115 USA
关键词
Angiogenesis; Matrigel; CD31; CD45; MADCAM1; Endothelial cells; ENDOTHELIAL GROWTH-FACTOR; ASSAYS; MODELS;
D O I
10.1016/j.jim.2008.11.016
中图分类号
Q5 [生物化学];
学科分类号
070307 [化学生物学];
摘要
Many of the current in vivo methods to evaluate angiogenesis are poorly quantifiable. Recently, the Matrigel plug assay has become the method of choice in many studies involving in vivo testing for angiogenesis. When known angiogenic factors are mixed with Matrigel and injected subcutaneously into mice, endothelial cells migrate into the gel plug. These endothelial cells form vessel-like structures, a process that mimics the formation of capillary networks. Here, we present a modification of the traditional Matrigel assay with improved method to quantify the amount of endothelial cells that incorporate into the plug. The removed plugs were subjected to a mild protease treatment, yielding intact cells. The liberated cells were then stained using an endothelial cell-specific markers, and counted by flow cytometry. This novel combination of FACS analysis with the traditional Matrigel assay improves the ability to quantify in vivo angiogenesis, and for the first time enables to determine the number of migrating and proliferating endothelial cells which reflects the angiogenesis rate. Published by Elsevier B.V.
引用
收藏
页码:78 / 81
页数:4
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