Microcalorimetric studies on the promoter function in E-coli TG1 from P-maltophilia AT18 chromosome DNA

被引：15

作者：

Abderrahmane, A

Yi, L

Wen-Ying, G

Ping, S ^{[1
]}

Song-Sheng, Q

机构：

[1] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Dept Chem, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

来源：

JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY | 2002年 / 68卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Escherichia coli TG(1); microcalorimetry; plasmid pKK232-8; promoter function; Pseudomonas maltophilia AT18;

D O I：

10.1023/A:1016138505617

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

In this paper, a promoter-probe plasmid pKK232-8 was used as a vector, which functioned in Escherichia coli TG(1) host. The plasmid DNA fragments from Pseudomonas maltophilia AT18 chromosome DNA active as promoter in Escherichia coli TG1, the promoter function was studied by means of microcalorimetry, the promoter is about 800 bp DNA, it can promote the chloramphenicol ( Cm) gene in plasmid pKK232-8, the Cm resistance level is about 80 mug mL(-1), the promoter activity is high. It implicates that there are probably many promoters in Pseudomonas maltophilia AT18 chromosome. All these information is readily obtained by an LKB 2277-204 heat conduction microcalorimeter. Microcalorimetry is a quantitative, inexpensive, and versatile method for microbiological genetic research.

引用

页码：909 / 916

页数：8

共 8 条

[1]

PLASMID VECTORS FOR THE SELECTION OF PROMOTERS [J].