Myeloid-Related Protein-14/MRP-14/S100A9/Calgranulin B is Associated with Inflammation in Proliferative Diabetic Retinopathy

被引：30

作者：

Abu El-Asrar, Ahmed M. ^{[1
,2
]}

Alam, Kaiser ^{[1
]}

Siddiquei, Mohammad M. ^{[1
]}

Van den Eynde, Kathleen ^{[3
]}

Mohammad, Ghulam ^{[1
]}

De Hertogh, Gert ^{[3
]}

Opdenakker, Ghislain ^{[4
]}

机构：

[1] King Saud Univ, Coll Med, Dept Ophthalmol, Riyadh, Saudi Arabia

[2] Dr Nasser Al Rashid Res Chair Ophthalmol, Riyadh, Saudi Arabia

[3] Univ Leuven, KU Leuven, Lab Histochem & Cytochem, Leuven, Belgium

[4] Univ Leuven, KU Leuven, Dept Microbiol & Immunol, Rega Inst Med Res,Lab Immunobiol, Leuven, Belgium

来源：

OCULAR IMMUNOLOGY AND INFLAMMATION | 2018年 / 26卷 / 04期

关键词：

Inflammation; leukocytes; myeloid-related proteins 8 and 14; proliferative diabetic retinopathy; proliferative vitreoretinopathy; ENDOTHELIAL-CELLS; S100A9; EXPRESSION; ANGIOGENESIS; ACTIVATION; PROTEINS; PATHWAY; MRP14; FIBROCYTES; HEPARANASE;

D O I：

10.1080/09273948.2016.1245759

中图分类号：

R77 [眼科学];

学科分类号：

100212 [眼科学];

摘要：

Purpose: To investigate the expression of the leukocyte proteins myeloid-related protein (MRP)-8 and MRP-14 in proliferative diabetic retinopathy (PDR) and the effect of MRP-8/MRP-14 (calprotectin) heterodimer on induction of proinflammatory factors in human retinal microvascular endothelial cells (HRMEC).Methods: Epiretinal membranes from 20 patients with PDR and 10 patients with proliferative vitreoretinopathy (PVR), vitreous fluid samples from PDR and non-diabetic subjects and HRMEC were studied by immunohistochemistry and Western blot analysis.Results: MRP-14 expression was localized in endothelial cells, leukocytes and myofibroblasts in all PDR membranes. MRP-8 expression was limited to intravascular leukocytes in 42% of the studied membranes. In PVR membranes, MRP-14 was expressed in leukocytes and myofibroblasts, whereas MRP-8 immunoreactivity was limited to leukocytes. MRP-14 was significantly upregulated in vitreous from PDR patients. MRP-8/MRP-14 (calprotectin) increased expression of intercellular adhesion molecule-1, but attenuated vascular cell adhesion molecule-1 expression in HRMEC.Conclusions: Increased MRP-14 levels are associated with inflammation in PDR.

引用

页码：615 / 624

页数：10

共 45 条

[1]

Circulating fibrocytes contribute to the myofibroblast population in proliferative vitreoretinopathy epiretinal membranes [J].

Abu El-Asrar, A. M. ;

Struyf, S. ;

Van Damme, J. ;

Geboes, K. .

BRITISH JOURNAL OF OPHTHALMOLOGY, 2008, 92 (05) :699-704

[2]

Abu El-Asrar AM, 2016, MOL VIS, V22, P424

[3]

Upregulated Expression of Heparanase in the Vitreous of Patients With Proliferative Diabetic Retinopathy Originates From Activated Endothelial Cells and Leukocytes [J].

Abu El-Asrar, Ahmed M. ;

Alam, Kaiser ;

Nawaz, Mohd Imtiaz ;

Mohammad, Ghulam ;

Van den Eynde, Kathleen ;

Siddiquei, Mohammad Mairaj ;

Mousa, Ahmed ;

De Hertogh, Gert ;

Geboes, Karel ;

Opdenakker, Ghislain .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2015, 56 (13) :8239-8247

[4]

Myofibroblasts in proliferative diabetic retinopathy can originate from infiltrating fibrocytes and through endothelial-to-mesenchymal transition (EndoMT) [J].

Abu El-Asrar, Ahmed M. ;

De Hertogh, Gert ;

van den Eynde, Kathleen ;

Alam, Kaiser ;

Van Raemdonck, Katrien ;

Opdenakker, Ghislain ;

Van Damme, Jo ;

Geboes, Karel ;

Struyf, Sofie .

EXPERIMENTAL EYE RESEARCH, 2015, 132 :179-189

[5]

Abu El-Asrar AM, 2014, MOL VIS, V20, P1209

[6]

Relationship between Vitreous Levels of Matrix Metalloproteinases and Vascular Endothelial Growth Factor in Proliferative Diabetic Retinopathy [J].

Abu El-Asrar, Ahmed M. ;

Mohammad, Ghulam ;

Nawaz, Mohd Imtiaz ;

Siddiquei, Mohammad Mairaj ;

Van den Eynde, Kathleen ;

Mousa, Ahmed ;

De Hertogh, Gert ;

Opdenakker, Ghislain .

PLOS ONE, 2013, 8 (12)

[7]

Angiogenic and Vasculogenic Factors in the Vitreous from Patients with Proliferative Diabetic Retinopathy [J].

Abu El-Asrar, Ahmed M. ;

Nawaz, Mohd Imtiaz ;

Kangave, Dustan ;

Siddiquei, Mohammed Mairaj ;

Geboes, Karel .

JOURNAL OF DIABETES RESEARCH, 2013, 2013

[8]

Abu El-Asrar AM, 2011, MOL VIS, V17, P508

[9]

Expression of myofibroblast activation molecules in proliferative vitreoretinopathy epiretinal membranes [J].

Abu El-Asrar, Ahmed M. ;

Missotten, Luc ;

Geboes, Karel .

ACTA OPHTHALMOLOGICA, 2011, 89 (02) :E115-E121

[10]

Expression of apoptosis markers in the retinas of human subjects with diabetes [J].

Abu El-Asrar, AM ;

Dralands, L ;

Missotten, L ;

Al-Jadaan, I ;

Geboes, K .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2004, 45 (08) :2760-2766

← 1 2 3 4 5 →