Structural analyses of new tri- and tetrasaccharides produced from disaccharides by transglycosylation of purified Trichoderma viride β-glucosidase

被引：26

作者：

Kono, H ^{[1
]}

Kawano, S ^{[1
]}

Tajima, K ^{[1
]}

Erata, T ^{[1
]}

Takai, M ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Mol Chem, Sapporo, Hokkaido 0608628, Japan

来源：

GLYCOCONJUGATE JOURNAL | 1999年 / 16卷 / 08期

关键词：

Trichoderma viride; beta-glucosidase; transglycosylation; two-dimensional NMR spectroscopy; oligosaccharide synthesis;

D O I：

10.1023/A:1007034728857

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new beta-glucosidase was partially purified from Trichoderma viride cellulase. This beta-glucosidase catalyzed a transglycosylation reaction of cellobiose to give beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 4)-D-Glc (1, yield: 18.8%) and beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 4)-D-Glc (2, 3.7%), regioselectively. Furthermore, the enzyme regioselectively converted laminaribiose and gentiobiose into beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 3)-D-Glc (3, 15.3%) and beta-D-Glc-(1 --> 6)-beta-D-Glc-(1 --> 6)-D-Glc (4, 20.2%), respectively. The structures (1-4) of the products were determined by H-1 and C-13 NMR spectroscopies. This high regio- and stereoselectively of the beta-glucosidase could be applied for oligosaccharide synthesis. [GRAPHICS]

引用

页码：415 / 423

页数：9

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