Nitrate interference with potassium-selective microelectrodes

被引：11

作者：

Cuin, TA ^{[1
]}

Miller, AJ ^{[1
]}

Laurie, SA ^{[1
]}

Leigh, RA ^{[1
]}

机构：

[1] AFRC, Inst Arable Crops Res, Dept Biochem & Physiol, Harpenden AL5 2JQ, Herts, England

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1999年 / 50卷 / 340期

基金：

英国生物技术与生命科学研究理事会;

关键词：

leaf epidermis; potassium activity; potassium-selective microelectrodes; vacuoles; nitrate interference;

D O I：

10.1093/jexbot/50.340.1709

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Initial attempts to measure K+ activity (a(k)) in vacuoles of barley leaf epidermal cells using triple-barrelled K+-Selective microelectrodes gave values that were only about one-third of those expected. This was due to high (c. 200 mM) NO3- concentrations in the vacuoles interfering with the K+-sensor, The effect of NO3- was on 1,2,-dimethyl-3-nitrobenzene (DNB) used as a plasticizer in the K+-sensor. Replacing DNB with dibutyl sebacate, but not with 2-nitrophenyl octyl ether, overcame this problem and the modified sensor gave acceptable calibration curves with no interference from physiological concentrations of other ions, These electrodes were used successfully to measure a mean a(k) of 235 mM in vacuoles of epidermal cells of K+-replete barley leaves.

引用

页码：1709 / 1712

页数：4

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