Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

被引：33

作者：

Campbell, SC ^{[1
]}

Cragg, H ^{[1
]}

Elrick, LJ ^{[1
]}

Macfarlane, WM ^{[1
]}

Shennan, KIJ ^{[1
]}

Docherty, K ^{[1
]}

机构：

[1] Univ Aberdeen, Inst Med Sci, Dept Mol & Cell Biol, Aberdeen AB25 2ZD, Scotland

来源：

FEBS LETTERS | 1999年 / 463卷 / 1-2期

基金：

英国惠康基金;

关键词：

insulin gene; diabetes mellitus; amylin; transcription;

D O I：

10.1016/S0014-5793(99)01584-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.

引用

页码：53 / 57

页数：5

共 23 条

[1]

BOAM DSW, 1990, J BIOL CHEM, V265, P8285

[2] Identification of cis- and trans-active factors regulating human islet amyloid polypeptide gene expression in pancreatic beta-cells [J].

Carty, MD ;

Lillquist, JS ;

Peshavaria, M ;

Stein, R ;

Soeller, WC .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (18) :11986-11993

[3] PAX-3 CONTAINS DOMAINS FOR TRANSCRIPTION ACTIVATION AND TRANSCRIPTION INHIBITION [J].

CHALEPAKIS, G ;

JONES, FS ;

EDELMAN, GM ;

GRUSS, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) :12745-12749

[4] C-terminal activating and inhibitory domains determine the transactivation potential of BSAP (Pax-5), Pax-2 and Pax-8 [J].

Dorfler, P ;

Busslinger, M .

EMBO JOURNAL, 1996, 15 (08) :1971-1982

[5]

EPSTEIN J, 1994, J BIOL CHEM, V269, P8355

[6] PAX IN DEVELOPMENT [J].

GRUSS, P ;

WALTHER, C .

CELL, 1992, 69 (05) :719-722

[7] PANCREATIC BETA-CELL LINE MIN6 EXHIBITS CHARACTERISTICS OF GLUCOSE-METABOLISM AND GLUCOSE-STIMULATED INSULIN-SECRETION SIMILAR TO THOSE OF NORMAL ISLETS [J].

ISHIHARA, H ;

ASANO, T ;

TSUKUDA, K ;

KATAGIRI, H ;

INUKAI, K ;

ANAI, M ;

KIKUCHI, M ;

YAZAKI, Y ;

MIYAZAKI, JI ;

OKA, Y .

DIABETOLOGIA, 1993, 36 (11) :1139-1145

[8]

Jun S, 1996, DEVELOPMENT, V122, P2639

[9] Molecular cloning of mouse paired-box-containing gene (Pax)-4 from an islet β cell line and deduced sequence of human Pax-4 [J].

Matsushita, T ;

Yamaoka, T ;

Otsuka, S ;

Moritani, M ;

Matsumoto, T ;

Itakura, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 242 (01) :176-180

[10] Characterization of a novel glucose-responsive insulin-secreting cell line, BRIN-BD11, produced by electrofusion [J].

McClenaghan, NH ;

Barnett, CR ;

AhSing, E ;

AbdelWahab, YH ;

OHarte, FPM ;

Yoon, TW ;

SwanstonFlatt, SK ;

Flatt, PR .

DIABETES, 1996, 45 (08) :1132-1140

← 1 2 3 →