Is whole-culture synchronization biology's 'perpetual-motion machine'?

被引：15

作者：

Cooper, S ^{[1
]}

机构：

[1] Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2004年 / 22卷 / 06期

关键词：

D O I：

10.1016/S0167-7799(04)00114-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Whole-culture or batch synchronization cannot, in theory, produce a synchronized culture because it violates a fundamental law that proposes that no batch treatment can alter the cell-age order of a culture. In analogy with the history of perpetual-motion machines, it is suggested that the study of these whole-culture 'synchronization' methods might lead to an understanding of general biological principles even though these methods cannot be used to study the normal cell cycle.

引用

页码：266 / 269

页数：4

共 17 条

[1] Transcriptional regulation and function during the human cell cycle [J].

Cho, RJ ;

Huang, MX ;

Campbell, MJ ;

Dong, HL ;

Steinmetz, L ;

Sapinoso, L ;

Hampton, G ;

Elledge, SJ ;

Davis, RW ;

Lockhart, DJ .

NATURE GENETICS, 2001, 27 (01) :48-54

[2] Reappraisal of serum starvation, the restriction point, G0, and G1 phase arrest points [J].

Cooper, S .

FASEB JOURNAL, 2003, 17 (03) :333-340

[3] Reappraisal of G1-phase arrest and synchronization by lovastatin [J].

Cooper, S .

CELL BIOLOGY INTERNATIONAL, 2002, 26 (08) :715-727

[4] Rethinking synchronization of mammalian cells for cell cycle analysis [J].

Cooper, S .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2003, 60 (06) :1099-1106

[5] Minimally disturbed, multicycle, and reproducible synchrony using a eukaryotic "baby machine" [J].

Cooper, S .

BIOESSAYS, 2002, 24 (06) :499-501

[6] The Schaechter-Bentzon-Maaloe experiment and the analysis of cell cycle events in eukaryotic cells [J].

Cooper, S .

TRENDS IN MICROBIOLOGY, 2002, 10 (04) :169-173

[7] Revisiting retinoblastoma protein phosphorylation during the mammalian cell cycle [J].

Cooper, S ;

Shayman, JA .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2001, 58 (04) :580-595

[8]

Cooper S, 2000, Prog Cell Cycle Res, V4, P27

[9] Mammalian cells are not synchronized in G1-phase by starvation or inhibition:: considerations of the fundamental concept of G1-phase synchronization [J].

Cooper, S .

CELL PROLIFERATION, 1998, 31 (01) :9-16

[10]

COOPER S, 1991, BACTERIAL GROWTH DIV

← 1 2 →