CD2 ligation abrogates antigen-independent apoptosis in B cells

被引:3
作者
Baixeras, E
Moreno, MC
MartinezA, C
机构
[1] Ctro. Nac. de Biotecnologia, Univ. Autónoma de Madrid, Campus de Cantoblanco
关键词
D O I
10.1006/cimm.1996.0172
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have described recently the prevention of apoptosis by CDS-soluble CD48 interaction on antigen B cell receptor occupancy. Here, we show that CD2 ligation is also able to interfere with B cell receptor-independent apoptosis pathways such as spontaneous death in spleen B cells or serum deprivation and hydrogen peroxide exposure in the BAL-17 cell line. In all cases, CD2 ligation induces a signal that prevents the downregulation of Bcl-2 expression. The specific CD2 signal pathway involved in this phenomenon is still unknown. As reported, CD2 did not appear to induce Ca2+ mobilization, phosphatidylinositol turnover, or PKC translocation in B cells. Nevertheless, we show that CD2 receptor ligation is coupled to the tyrosine phosphorylation pathway in B cells. These observations indicate that CD2 is functionally able to trigger at least an early signal that could play a role in apoptosis blockage B cells in addition to the adhesion one. The results suggest the participation of cellular membrane receptors other that CD40 in apoptosis rescue, not only in the antigen-dependent but also in the antigen-independent phases of B cell lymphopoiesis. (C) 1996 Academic Press, Inc.
引用
收藏
页码:55 / 61
页数:7
相关论文
共 46 条
[1]   A SOLUBLE MULTIMERIC RECOMBINANT CD2 PROTEIN IDENTIFIES CD48 AS A LOW AFFINITY LIGAND FOR HUMAN CD2 - DIVERGENCE OF CD2 LIGANDS DURING THE EVOLUTION OF HUMANS AND MICE [J].
ARULANANDAM, ARN ;
MOINGEON, P ;
CONCINO, MF ;
RECNY, MA ;
KATO, K ;
YAGITA, H ;
KOYASU, S ;
REINHERZ, EL .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (05) :1439-1450
[2]   FROM APOPTOSIS TO AUTOIMMUNITY - INSIGHTS FROM THE SIGNALING PATHWAYS LEADING TO PROLIFERATION OR TO PROGRAMMED CELL-DEATH [J].
BAIXERAS, E ;
BOSCA, L ;
STAUBER, C ;
GONZALEZ, A ;
CARRERA, AC ;
GONZALO, JA ;
MARTINEZA, C .
IMMUNOLOGICAL REVIEWS, 1994, 142 :53-91
[3]   SIGNAL-TRANSDUCTION PATHWAYS INVOLVED IN B-CELL INDUCTION [J].
BAIXERAS, E ;
KROEMER, G ;
CUENDE, E ;
MARQUEZ, C ;
BOSCA, L ;
MARTINEZ, JEA ;
MARTINEZ, AC .
IMMUNOLOGICAL REVIEWS, 1993, 132 :5-47
[4]  
BAIXERAS E, IN PRESS SCAND J IMM
[5]   BCL-X, A BCL-2-RELATED GENE THAT FUNCTIONS AS A DOMINANT REGULATOR OF APOPTOTIC CELL-DEATH [J].
BOISE, LH ;
GONZALEZGARCIA, M ;
POSTEMA, CE ;
DING, LY ;
LINDSTEN, T ;
TURKA, LA ;
MAO, XH ;
NUNEZ, G ;
THOMPSON, CB .
CELL, 1993, 74 (04) :597-608
[6]   THYMOCYTE APOPTOSIS INDUCED BY P53-DEPENDENT AND INDEPENDENT PATHWAYS [J].
CLARKE, AR ;
PURDIE, CA ;
HARRISON, DJ ;
MORRIS, RG ;
BIRD, CC ;
HOOPER, ML ;
WYLLIE, AH .
NATURE, 1993, 362 (6423) :849-852
[7]  
COHEN JJ, 1993, IMMUNOL TODAY, V14, P126, DOI 10.1016/0167-5699(93)90214-6
[8]  
DENNING SM, 1988, J IMMUNOL, V141, P2980
[9]   TRANSCRIPTIONAL TRANSACTIVATION FUNCTIONS LOCALIZED TO THE GLUCOCORTICOID RECEPTOR-N TERMINUS ARE NECESSARY FOR STEROID INDUCTION OF LYMPHOCYTE APOPTOSIS [J].
DIEKEN, ES ;
MIESFELD, RL .
MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (02) :589-597
[10]   CD2 - A FUNCTIONAL ADHESION MOLECULE ON MURINE B-CELLS, INVOLVED IN INTERLEUKIN-4-INDUCED AGGREGATION [J].
ELENSTROMMAGNUSSON, C ;
ALTEVOGT, P ;
SEVERINSON, E .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (04) :888-892