Exercise preconditioning against hydrogen peroxide-induced oxidative damage in proteins of rat myocardium

被引:62
作者
Radák, Z
Sasvári, M
Nyakas, C
Pucsok, J
Nakamoto, H
Goto, S
机构
[1] Semmelweis Univ, Exercise Physiol Lab, Sch Sport Sci, Budapest, Hungary
[2] Semmelweis Univ, Sch Hlth Sci, Cent Lab, Budapest, Hungary
[3] Toho Univ, Sch Pharmaceut Sci, Dept Biochem, Funabashi, Chiba 274, Japan
关键词
D O I
10.1006/abbi.2000.1719
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Both regular physical exercise and low levels of H2O2 administration result in increased resistance to oxidative stress. We measured the accumulation of reactive carbonyl derivatives and the activities of proteasome complex and DT-diaphorase in cardiac muscle of trained and untrained rats after chronic i.p, administration of 1 ml t-butyl H2O2 (1 mmol/kg for 3 weeks every second day). Twenty-four rats were randomly assigned to a control group administered with saline, control administered with H2O2, and exercised administered either saline or H2O2. The activity of DT-diaphorase significantly increased in H2O2 administered and exercised groups, indicating that an increase in H2O2 levels stimulate the activity of this enzyme. The cardiac muscle of H2O2 administered nonexercised animals accumulated significantly more carbonyl than control group (P < 0.05), The exercise and H2O2 administration resulted in less oxidatively modified protein than found in nonexercised groups (P < 0.05). The peptide-like activity of proteasome complex was induced by the treatment of H2O2 and exercise and exercise potentiate the effect of H2O2. On the other hand, the chymotrypsin-like and trypsin-like activities were stimulated only by physical training and H2O2 administration. The data suggest that chronic administration of H2O2 after exercise training decreases the accumulation of carbonyl groups below the steady-state level and induces the activity of proteasome and DT-diaphorase. Hence, the stimulating effect of physical exercise on free radical generation is an important phenomenon of the exercise-induced adaptation process since it increases resistance to oxidative stress. Regular exercise training is a valuable physiological means of preconditioning the myocardium to prolonged oxidative stress. (C) 2000 Academic Press.
引用
收藏
页码:248 / 251
页数:4
相关论文
共 28 条
[1]  
Astrand P. O., 1986, TXB WORK PHYSL
[2]  
AVERSA P, 1987, CIRCULATION, V75, P112
[3]   Modulation of a cardiogenic shock inducible RNA by chemical stress: adapt73/PigHep3 [J].
Crawford, DR ;
Davies, KJA .
SURGERY, 1997, 121 (05) :581-587
[4]  
DAVIES KJA, 1987, J BIOL CHEM, V262, P9914
[5]  
ERSTNER L, 1987, CHEM SCRIPTA, V27, P1
[6]  
ERSTNER L, 1967, METHOD ENZYMOL, V10, P309
[7]  
ERSTNER L, 1995, BIOCHIM BIOPHYS ACTA, V1271, P195
[8]   Degradation of oxidized proteins in K562 human hematopoietic cells by proteasome [J].
Grune, T ;
Reinheckel, T ;
Davies, KJA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (26) :15504-15509
[9]   Age-related changes in the 20S and 26S proteasome activities in the liver of male F344 rats [J].
Hayashi, T ;
Goto, S .
MECHANISMS OF AGEING AND DEVELOPMENT, 1998, 102 (01) :55-66
[10]   CARDIAC-HYPERTROPHY ALTERS MYOCARDIAL RESPONSE TO ISCHEMIA AND REPERFUSION IN-VIVO [J].
JI, LL ;
FU, RG ;
MITCHELL, EW ;
GRIFFITHS, M ;
WALDROP, TG ;
SWARTZ, HM .
ACTA PHYSIOLOGICA SCANDINAVICA, 1994, 151 (03) :279-290