Genesis, a winged helix transcriptional repressor with expression restricted to embryonic stem cells

被引:156
作者
Sutton, J
Costa, R
Klug, M
Field, L
Xu, DW
Largaespada, DA
Fletcher, CF
Jenkins, NA
Copeland, NG
Klemsz, M
Hromas, R
机构
[1] INDIANA UNIV,MED CTR,DIV HEMATOL ONCOL,INDIANAPOLIS,IN 46202
[2] INDIANA UNIV,MED CTR,WALTHER ONCOL CTR,INDIANAPOLIS,IN 46202
[3] UNIV ILLINOIS,COLL MED,DEPT BIOCHEM,CHICAGO,IL 60612
[4] INDIANA UNIV,MED CTR,KRANNERT INST CARDIOL,INDIANAPOLIS,IN 46202
[5] NCI,MAMMALIAN GENET LAB,ABL BASIC RES PROGRAM,NIH,FREDERICK CANC RES & DEV CTR,FREDERICK,MD 21702
[6] INDIANA UNIV,MED CTR,DEPT MICROBIOL & IMMUNOL,INDIANAPOLIS,IN 46202
关键词
D O I
10.1074/jbc.271.38.23126
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A novel member of the winged helix (formerly HNF-3/Forkhead) transcriptional regulatory family, termed Genesis, was isolated and characterized. Putative translation of the complete cDNA revealed the winged helix DNA binding domain to be centrally located within the protein, with regions on either side that contain known transcriptional regulatory motifs. Extensive Northern analysis of Genesis found that the message was exclusively expressed in embryonic stem cells or their malignant equivalent, embryonal carcinoma cells. The Genesis transcript was down-regulated when these cells were stimulated to differentiate. DNA sequences that Genesis protein would interact with were characterized and were found to contain a consensus similar to that found in an embryonic stem cell enhancer sequence, Go-transfection experiments revealed that Genesis is a transcriptional repressor. Genesis mapped to mouse chromosome 4 in a region syntenic with human chromosome 1p31, a site of nonrandom abnormalities in germ cell neoplasia, neuroblastoma, and acute lymphoblastic leukemia, Genesis is a candidate for regulating the phenotype of normal or malignant embryonic stem cells.
引用
收藏
页码:23126 / 23133
页数:8
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