Synthesis and structural characterization of poly(LGGVG), an elastin-like polypeptide

Poly(LGGVG) a potential elastin-like biomaterial has been synthesized and studied both in solution (by circular dicroism and nuclear magnetic resonance) and in the aggregated state (by transmission electron microscopy). For sake of comparison, also the conformation of the protected (Boc-LGGVG-OEt) and free (H-2(+)-LGGVG-OH) 'monomers' has been investigated. While in the latter ones the presence has been evidenced of more or less stable type LI beta-turns, the polymer showed a conformational ensemble, possibly comprising type II beta-turns, type I beta-turns and open (unordered) structures. At supramolecular level, twisted-rope aggregates were observed by transmission electron microscopy for the polymer. Thus, the title compound has shown to possess, at both molecular and supramolecular level, physico-chemical properties very similar to those of elastin, so to give some confidence that it could really constitute the precursor of an artificial substitute of elastin itself. (C) 2000 Published by Elsevier Science B.V. All rights reserved.