Novel and complex chromosomal arrangement of Rhizobium loti nodulation genes

被引：31

作者：

Scott, DB ^{[1
]}

Young, CA ^{[1
]}

CollinsEmerson, JM ^{[1
]}

Terzaghi, EA ^{[1
]}

Rockman, ES ^{[1
]}

Lewis, PE ^{[1
]}

Pankhurst, CE ^{[1
]}

机构：

[1] DSIR, DIV BIOTECHNOL, PALMERSTON NORTH, NEW ZEALAND

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1996年 / 9卷 / 03期

关键词：

nodulation gene rearrangements;

D O I：

10.1094/MPMI-9-0187

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mutational and structural analysis of Rhizobium loti nodulation genes in strains NZP2037 and NZP2213 was carried out. Unlike the case with other Rhizobium strains examined to date, nodB was found on an operon separate from nodACIJ. Sequence analysis of the nodACIJ and nodB operon regions confirm that R. loti common nod genes have a gene organization different from that of other Rhizobium spp. At least 4 copies of nodD-like sequences were identified in R. loti. The complete nucleotide sequence of one of these, nodD3, was determined, A new host-specific nod gene, nolL, was identified adjacent to nodD3, NolL, shares homology with NodX and other O-acetyl transferases. Mutational analysis of the nod regions of strains NZP2037 and NZP2213 showed that nodD3, nodI, nodJ, and nolL were all essential for R. loti strains to effectively nodulate the extended host Lotus pedunculatus, but were not necessary for effective nodulation of the less restrictive host, Lotus corniculatus. Both nodD3 and nolL were essential for R. loti strains to nodulate Leucaena leucocephala.

引用

页码：187 / 197

页数：11

共 82 条

[1]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[2] RHIZOBIUM-JAPONICUM USDA 191 HAS 2 NODD GENES THAT DIFFER IN PRIMARY STRUCTURE AND FUNCTION [J].