Guidelines for validation of qualitative real-time PCR methods

被引：340

作者：

Broeders, S. ^{[1
]}

Huber, I. ^{[2
]}

Grohmann, L. ^{[3
]}

Berben, G. ^{[4
]}

Taverniers, I. ^{[5
]}

Mazzara, M. ^{[6
]}

Roosens, N. ^{[1
]}

Morisset, D. ^{[7
]}

机构：

[1] Sci Inst Publ Hlth WIV ISP, B-1050 Brussels, Belgium

[2] Bavarian Hlth & Food Safety Author LGL, D-85764 Oberschleissheim, Germany

[3] Fed Off Consumer Protect & Food Safety BVL, D-10117 Berlin, Germany

[4] Walloon Agr Res Ctr CRA W, B-5030 Gembloux, Belgium

[5] Inst Agr & Fisheries Res EV ILVO, B-9820 Merelbeke, Belgium

[6] European Commiss, Directorate Gen Joint Res Ctr, Inst Hlth & Consumer Protect EC JRC IHCP, I-21027 Ispra, VA, Italy

[7] Natl Inst Biol, Ljubljana 1000, Slovenia

来源：

TRENDS IN FOOD SCIENCE & TECHNOLOGY | 2014年 / 37卷 / 02期

关键词：

GENETICALLY-MODIFIED ORGANISMS; MINIMUM INFORMATION; SCREENING METHODS; REFERENCE GENE; PUBLICATION; DUPLEX; ASSAYS; FOOD;

D O I：

10.1016/j.tifs.2014.03.008

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

As for many areas of molecular testing, detection of Genetically Modified Organisms (GMO) relies on the real-time Polymerase Chain Reaction (qPCR) technology. Due to the increasing number of GMO, a screening approach using qualitative screening methods has become an integrated part of GMO detection. However, specific guidelines for the validation of these methods are lacking. Here, a pragmatic approach to conduct in-house and inter-laboratory validation studies for GMO screening methods, is proposed. Such guidelines could be adapted to other areas where qualitative qPCR methods are used for molecular testing allowing to implement easily a more reliable screening phase where necessary.

引用

页码：115 / 126

页数：12

共 50 条

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