Inhibitors of the LPS-induced NF-κB activation from Artemisia sylvatica

被引:37
作者
Hui, ZJ
Lee, JH
Lee, D
Hong, YS
Kim, YH
Lee, JJ
机构
[1] Korea Res Inst Biosci & Biotechnol, Anticanc Res Lab, Taejon 305600, South Korea
[2] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea
关键词
Artemisia sylvatica; Compositae; sesquiterpene lactone; NF-kappa B; NO; TNF-alpha; iNOS; COX-2;
D O I
10.1016/j.phytochem.2004.06.034
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three guaianolide sesquiterpene lactones, 3alpha,4alpha-epoxyrupicolins C-E, together with six known sesquiterpenes, artemisolide, 3-methoxytanapartholide, deacetyllaurenobiolide, moxartenolide as well as arteminolides B and D were isolated by bioassay-guided fractionation from the methanol extract of the aerial parts of Artemisia sylvatica using the NF-kappaB mediated reporter gene assay. All isolated compounds displayed inhibitory activity on the LPS-induced NF-kappaB activation, NO production, and TNF-alpha production with IC50 values of 0.49-7.17, 1.46-6.16, and 3.19-27.76 muM, respectively, in RAW264.7 cells. It was also established that arteminolide B suppressed the expression of NF-kappaB target genes such as iNOS and COX-2. This is the first report of NF-kappaB inhibitory activities of these compounds and supports the pharmacological use of Artemisia sylvatica, which has been employed as an herbal medicine for the treatment of inflammation. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:2247 / 2253
页数:7
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