Recycling electrophoretic separations: Modeling of isotachophoresis and isoelectric focusing

被引:14
作者
Baygents, JC
Schwarz, BCE
Deshmukh, RR
Bier, M
机构
[1] Dept. of Chem. and Environ. Eng., University of Arizona, Tucson
关键词
isotachophoresis; isoelectric focusing; simulated electrophoresis; recycling electrophoresis; proteins; albumin; haemoglobin;
D O I
10.1016/S0021-9673(96)00963-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The conventional, one-dimensional model that is the foundation for the dynamic simulation of electrophoretic separations is modified to describe the essential features of recycling electrophoresis instruments. Simulations are performed for the isoelectric focusing (IEF) and the isotachophoresis (ITP) modes. Results are compared with experimental data from prototype recycling free-flow IEF and ITP devices. Agreement between the experiments and the simulations is favorable, though electrode and electroosmotic effects, which are omitted from the model, are noticeable in the IEF instrument. Three parameters relevant to the design of recycling electrophoresis devices, viz. the number of ports for the recycle manifold, the residence time in the separation chamber per cycle, and the current density, are investigated with the model. (C) 1996 Elsevier Science B.V.
引用
收藏
页码:165 / 183
页数:19
相关论文
共 23 条
[1]   ELECTROPHORESIS - MATHEMATICAL-MODELING AND COMPUTER-SIMULATION [J].
BIER, M ;
PALUSINSKI, OA ;
MOSHER, RA ;
SAVILLE, DA .
SCIENCE, 1983, 219 (4590) :1281-1287
[2]   A NEW BUFFERING SYSTEM AND ITS USE IN ELECTROPHORESIS AND ISOELECTRIC-FOCUSING [J].
BIER, M ;
OSTREM, J ;
MARQUEZ, RB .
ELECTROPHORESIS, 1993, 14 (10) :1011-1018
[3]   RECYCLING ISOELECTRIC-FOCUSING AND ISOTACHOPHORESIS [J].
BIER, M ;
TWITTY, GE ;
SLOAN, JE .
JOURNAL OF CHROMATOGRAPHY, 1989, 470 (02) :369-376
[4]   COMPUTER-SIMULATION AND EXPERIMENTAL VALIDATION OF ISOELECTRIC-FOCUSING IN AMPHOLINE-FREE SYSTEMS [J].
BIER, M ;
MOSHER, RA ;
PALUSINSKI, OA .
JOURNAL OF CHROMATOGRAPHY, 1981, 211 (03) :313-335
[5]  
BIER M, 1979, PEPTIDES STRUCTURE B
[6]  
BIER M, 1984, ELECTROPHORESIS 83, P547
[7]  
Carnahan B., 1969, APPL NUMERICAL METHO
[8]   COUNTERFLOW IN ISOTACHOPHORESIS - COMPUTER-SIMULATION AND EXPERIMENTAL STUDIES [J].
DESHMUKH, RR ;
BIER, M .
ELECTROPHORESIS, 1993, 14 (03) :205-213
[9]  
Ermakov S., 1992, Informatica, V3, P173
[10]   NUMERICAL ALGORITHMS FOR CAPILLARY ELECTROPHORESIS [J].
ERMAKOV, SV ;
BELLO, MS ;
RIGHETTI, PG .
JOURNAL OF CHROMATOGRAPHY A, 1994, 661 (1-2) :265-278