An animal component free medium that promotes the growth of various animal cell lines for the production of viral vaccines

被引：22

作者：

Rourou, Samia ^{[1
]}

Ben Ayed, Yousr ^{[1
]}

Trabelsi, Khaled ^{[1
]}

Majoul, Samy ^{[1
]}

Kallel, Hela ^{[1
]}

机构：

[1] Inst Pasteur Tunis, Lab Mol Microbiol Vaccinol & Biotechnol Dev, Viral Vaccines Res & Dev Unit, Tunis 1002, Tunisia

来源：

VACCINE | 2014年 / 32卷 / 24期

关键词：

Animal component free medium; Vero cells; MRC-5; cells; BHK-21; Rabies virus; Measles viruses; STIRRED BIOREACTOR; CULTURE; VIRUS;

D O I：

10.1016/j.vaccine.2014.02.040

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

IPT-AFM is a proprietary animal component free medium that was developed for rabies virus (strain LP 2061) production in Vero cells. In the present work, we demonstrated the versatility of this medium and its ability to sustain the growth of other cell lines and different virus strains. Here, three models were presented: Vero cells/rabies virus (strain LP 2061), MRC-5 cells/measles virus (strain AIK-C) and BHK-21 cells/rabies virus (strain PV-BHK21). The cell lines were first adapted to grow in IPT-AFM, by progressive reduction of the amount of serum in the culture medium. After their adaptation, BHK-21 cells grew in suspension by forming clumps, whereas MRC-5 cells remained adherent. Then, kinetics of cell growth were studied in agitated cultures for both cell lines. In addition, kinetics of virus replication were investigated. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：2767 / 2769

页数：3

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