Evaluation of rapid molecular methods for detection of clarithromycin resistance in Helicobacter pylori

被引:37
作者
Szczebara, F
Dhaenens, L
Vincent, P
Husson, MO
机构
[1] Lab. de Bacteriol.-Hygiène, Faculté de Médecine, 59045 Lille Cedex
关键词
D O I
10.1007/BF01709478
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Resistance of Helicobacter pylori to clarithromycin is due to point mutations at position A2143 or A2144 of the rrnH 23S rRNA gene, each mutation creating an additional restriction site for BsaI or MboII. A procedure combining PGR and RFLP analysis was evaluated for detection of these mutations using primers specific for the 23S rRNA gene, and BsaI and MboII enzymes, All clarithromycin-resistant isolates (8/8), as defined by the MIG, were found to be resistant by PCR-RFLP. No clarithromycin-sensitive isolates (14/14) gave a positive reaction.
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页码:162 / 164
页数:3
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