βig-h3 supports keratinocyte adhesion, migration, and proliferation through α3β1 integrin

被引：132

作者：

Bae, JS

Lee, SH

Kim, JE

Choi, JY

Park, RW

Park, JY

Park, HS

Sohn, YS

Lee, DS

Lee, EB

Kim, IS ^{[1
]}

机构：

[1] Kyungpook Natl Univ, Sch Med, Cell & Matrix Natl Res Lab, Taegu 700422, South Korea

[2] Korea Canc Ctr Hosp, Seoul, South Korea

[3] Regen Biotech, Seoul, South Korea

[4] Korea Inst Sci & Technol, Biomed Res Ctr, Seoul 130650, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 294卷 / 05期

关键词：

beta ig-h3; alpha; 3; beta; 1; integrin; keratinocyte; wound healing; TGF-beta; cell adhesion; migration;

D O I：

10.1016/S0006-291X(02)00576-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

betaig-h3 is an extracellular matrix protein and its expression is highly induced by TGF-beta and it has also been suggested to play important roles in skin wound healing. In this paper, we demonstrate that betaig-h3 is present in the papillary layer of dermis and synthesized in the basal keratinocytes in vivo and its expression is induced by TGF-beta in normal human keratinocytes (NHEK) and HaCaT cells. betaig-h3 mediates not only adhesion and spreading of keratinocytes but also supports migration and proliferation. These activities are mediated through interacting with alpha3beta1 integrin. Previously identified two alpha3beta1 integrin-interacting motifs of betaig-M, EPDIM, and NKDIL, are responsible for these activities. The results suggest that betaig-h3 may regulate keratinocyte functions in normal skin and potentially during wound-healing process. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：940 / 948

页数：9

共 36 条

[1] alpha 3 beta 1 integrin is required for normal development of the epidermal basement membrane [J].