Syntheses of functionalized indium porphyrins for monoclonal antibody labeling

被引：10

作者：

BedelCloutour, CH

Mauclaire, L

Saux, A

Pereyre, M

机构：

[1] UNIV BORDEAUX 1,CHIM ORGAN & ORGANOMET LAB,URA 35,F-33405 TALENCE,FRANCE

[2] CIS BIOINT,F-91192 GIF SUR YVETTE,FRANCE

来源：

BIOCONJUGATE CHEMISTRY | 1996年 / 7卷 / 06期

关键词：

D O I：

10.1021/bc960045i

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The syntheses and characterizations of five differently hydrosoluble monosubstituted aryl porphyrins are reported. Their metallation with indium-lll was achieved and provided tracers with strong specific activities. The covalent coupling between indium-lll porphyrins and BSA served as a model reaction for the definition of the best experimental coupling conditions; the transposition to the labeling of anti-CEA monoclonal antibody was realized. The conjugates exhibited an in vitro good mAb-labeling efficiency, as well as a good preservation of immunoreactivity.

引用

页码：617 / 627

页数：11

共 46 条

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