Direct electrochemistry of hemoglobin in egg-phosphatidylcholine films and its catalysis to H2O2

被引：122

作者：

Han, XJ

Huang, WM

Jia, JB

Dong, SJ

Wang, EK ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Natl Analyt Res Ctr Electrochem & Spect, Changchun 130022, Jilin, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2002年 / 17卷 / 09期

基金：

中国国家自然科学基金;

关键词：

hemoglobin; egg-phosphaticylcholine vesicle dispersion; direct electrochemistry; H2O2;

D O I：

10.1016/S0956-5663(02)00052-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Direct electrochemistry of hemoglobin was observed in stable thin film composed of a natural lipid (egg-phosphatidylcholine) and hemoglobin on pyrolytic graphite (PG) electrode. Hemoglobin in lipid films shows thin layer electrochemistry behavior. The formal potential Edegrees' of hemoglobin in the lipid film was linearly varied with pH in the range from 3.5 to 7.0 with a slope of -46.4 mV pH(-1) Hemoglobin in the lipid film exhibited elegant catalytic activity for electrochemical reduction of H202, based which a unmediated biosensor for H2O2 was developed. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：741 / 746

页数：6

共 24 条

[1] DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS [J].