Microarray transcription profiling of a Shewanella oneidensis etrA mutant

被引:56
作者
Beliaev, AS
Thompson, DK
Fields, MW
Wu, LY
Lies, DP
Nealson, KH
Zhou, JZ
机构
[1] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA
[2] CALTECH, Div Geol & Planetary Sci, Pasadena, CA 91125 USA
[3] Univ So Calif, Los Angeles, CA 90089 USA
关键词
D O I
10.1128/JB.184.16.4612-4616.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA microarrays were used to examine the effect of an insertional mutation in the Shewanella oneidensis etrA (electron transport regulator) locus on gene expression under anaerobic conditions. The mRNA levels of 69 genes with documented functions in energy and carbon metabolism, regulation, transport, and other cellular processes displayed significant alterations in transcript abundance in an etr4-mutant genetic background. This is the first microarray study indicating a possible involvement of EtrA in the regulation of gene expression in S. oneidensis MR-1.
引用
收藏
页码:4612 / 4616
页数:5
相关论文
共 29 条
[1]   The pKNOCK series of broad-host-range mobilizable suicide vectors for gene knockout and targeted DNA insertion into the chromosome of Gram-negative bacteria [J].
Alexeyev, MF .
BIOTECHNIQUES, 1999, 26 (05) :824-+
[2]   MOLECULAR MECHANISM OF REGULATION OF SIDEROPHORE-MEDIATED IRON ASSIMILATION [J].
BAGG, A ;
NEILANDS, JB .
MICROBIOLOGICAL REVIEWS, 1987, 51 (04) :509-518
[3]   Mechanisms for redox control of gene expression [J].
Bauer, CE ;
Elsen, S ;
Bird, TH .
ANNUAL REVIEW OF MICROBIOLOGY, 1999, 53 :495-523
[4]  
Beliaev Alex S., 2002, OMICS A Journal of Integrative Biology, V6, P39, DOI 10.1089/15362310252780834
[5]  
Beliaev AS, 1998, J BACTERIOL, V180, P6292
[6]   Aerobic regulation of cytochrome d oxidase (cydAB) operon expression in Escherichia coli: Roles of Fnr and ArcA in repression and activation [J].
Cotter, PA ;
Melville, SB ;
Albrecht, JA ;
Gunsalus, RP .
MOLECULAR MICROBIOLOGY, 1997, 25 (03) :605-615
[7]   ANAEROBIC CONTROL OF DENITRIFICATION IN PSEUDOMONAS-STUTZERI ESCAPES MUTAGENESIS OF AN FNR-LIKE GENE [J].
CUYPERS, H ;
ZUMFT, WG .
JOURNAL OF BACTERIOLOGY, 1993, 175 (22) :7236-7246
[8]   The primary structure of the split-Soret cytochrome c from Desulfovibrio desulfuricans ATCC 27774 reveals an unusual type of diheme cytochrome c [J].
Devreese, B ;
Costa, C ;
Demol, H ;
Papaefthymiou, V ;
Moura, I ;
Moura, JJR ;
VanBeeumen, J .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 248 (02) :445-451
[9]   Cluster analysis and display of genome-wide expression patterns [J].
Eisen, MB ;
Spellman, PT ;
Brown, PO ;
Botstein, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) :14863-14868
[10]   REGULATION OF EXPRESSION OF THE CYTOCHROME-D TERMINAL OXIDASE IN ESCHERICHIA-COLI IS TRANSCRIPTIONAL [J].
GEORGIOU, CD ;
DUEWEKE, TJ ;
GENNIS, RB .
JOURNAL OF BACTERIOLOGY, 1988, 170 (02) :961-966