Affinity Purification of Trypsin Inhibitor with Anti-Aspergillus Flavus Activity from Cultivated and Wild Soybean

被引：8

作者：

Zhang, Bin ^{[1
]}

Wang, Dong-Feng ^{[1
]}

Fan, Yan ^{[1
]}

Zhang, Li ^{[1
]}

Luo, Yi ^{[1
]}

机构：

[1] Ocean Univ China, Dept Food Chem & Nutr, Coll Food Sci & Technol, Qingdao 266003, Peoples R China

来源：

MYCOPATHOLOGIA | 2009年 / 167卷 / 03期

关键词：

Aspergillus flavus; Chitosan resin; Affinity chromatography; Trypsin inhibitor; ANTIFUNGAL PROTEIN; MYCOTOXINS; CHITINASES; FUNGI;

D O I：

10.1007/s11046-008-9160-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Trypsin inhibitors (TI) from wild-type soybean (Glycine soya) (WBTI) and domesticated soybean (Glycine max) (SBTI) were purified using prepared chitosan resin-trypsin as filler on the affinity chromatography column. The SBTI/WBTI purification fold by affinity chromatography was 718- and 279-fold, with the activity recovery of 62% and 59%, respectively. It was found that SBTI and WBTI exerted a strong inhibition of Aspergillus. flavus growth, with IC50 of 1.6 and 1.0 mu mol/l. This growth inhibition was possibly the result of the inhibition on alpha-amylase activity of A. flavus by both the SBTI and WBTI. This was further supported by the fact that in the presence of SBTI and WBTI at 9.0 and 6.0 mu g/g (peanut) on peanuts inhibited the germination and growth of A. flavus. Accordingly, characterization of the mode of action of SBTI and WBTI could constitute a first step leading to resistance to A. flavus invasion.

引用

页码：163 / 171

页数：9

共 32 条

[1] Worldwide occurrence of mycotoxins in commodities, feeds and feed ingredients [J].

Binder, E. M. ;

Tan, L. M. ;

Chin, L. J. ;

Handl, J. ;

Richard, J. .

ANIMAL FEED SCIENCE AND TECHNOLOGY, 2007, 137 (3-4) :265-282

[2]

CARLTON B, 1961, ANAL BIOCHEM, V2, P370, DOI DOI 10.1016/0003-2697(61)90010-0

[3] ALPHA-AMYLASES FROM 3 SPECIES OF STORED GRAIN COLEOPTERA AND THEIR INHIBITION BY WHEAT AND CORN PROTEINACEOUS INHIBITORS [J].

CHEN, MS ;

FENG, GH ;

ZEN, KC ;

RICHARDSON, M ;

VALDESRODRIGUEZ, S ;

REECK, GR ;

KRAMER, KJ .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1992, 22 (03) :261-&

[4]

Chen Z.-Y., 1998, Phytopathology, V88, pS16

[5]

Chen ZY, 1999, APPL ENVIRON MICROB, V65, P1320

[6] A corn trypsin inhibitor with antifungal act inhibits Aspergillus flavus α-amylase [J].

Chen, ZY ;

Brown, RL ;

Russin, JS ;

Lax, AR ;

Cleveland, TE .

PHYTOPATHOLOGY, 1999, 89 (10) :902-907

[7] United States Department of Agriculture - Agricultural Research Service research on pre-harvest prevention of mycotoxins and mycotoxigenic fungi in US crops [J].

Cleveland, TE ;

Dowd, PF ;

Desjardins, AE ;

Bhatnagar, D ;

Cotty, PJ .

PEST MANAGEMENT SCIENCE, 2003, 59 (6-7) :629-642

[8] Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum [J].

Epple, P ;

Apel, K ;

Bohlmann, H .

PLANT CELL, 1997, 9 (04) :509-520

[9] Amy1, the α-amylase gene of Aspergillus flavus:: Involvement in aflatoxin biosynthesis in maize kernels [J].

Fakhoury, AM ;

Woloshuk, CP .

PHYTOPATHOLOGY, 1999, 89 (10) :908-914

[10] Some thaumatin-like proteins hydrolyse polymeric β-1,3-glucans [J].

Grenier, J ;

Potvin, C ;

Trudel, J ;

Asselin, A .

PLANT JOURNAL, 1999, 19 (04) :473-480

← 1 2 3 4 →