An endosymbiotic bacterium in a plant-parasitic nematode: Member of a new Wolbachia supergroup

被引：116

作者：

Haegeman, Annelies ^{[1
]}

Vanholme, Bartel ^{[1
]}

Jacob, Joachim ^{[1
]}

Vandekerckhove, Tom T. M. ^{[1
]}

Claeys, Myriam ^{[2
]}

Borgonie, Gaetan ^{[2
]}

Gheysen, Godelieve ^{[1
]}

机构：

[1] Univ Ghent, Fac Biosci Engn, Dept Mol Biotechnol, B-9000 Ghent, Belgium

[2] Univ Ghent, Fac Sci, Dept Biol, Nematol Unit, B-9000 Ghent, Belgium

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2009年 / 39卷 / 09期

关键词：

Radopholus similis; Endosymbiont; Rickettsiales; Expressed sequence tag; Bayesian phylogeny; Immunolocalisation; Transmission electron microscopy; MOLECULAR-IDENTIFICATION; PHYLOGENETIC ANALYSIS; BURROWING NEMATODE; TAXONOMIC STATUS; PIPIENTIS; EVOLUTION; SYMBIONTS; ABSENCE; GENOME; GENES;

D O I：

10.1016/j.ijpara.2009.01.006

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Wolbachia is an endosymbiotic bacterium widely present in arthropods and animal-parasitic nematodes. Despite previous efforts, it has never been identified in plant-parasitic nematodes. Random sequencing of genes expressed by the burrowing nematode Radopholus similis resulted in several sequences with similarity to Wolbachia genes. The presence of a Wolbachia-like endosymbiont in this plant-parasitic nematode was investigated using both morphological and molecular approaches. Transmission electron microscopy, fluorescent immunolocalisation and staining with DAPI confirmed the presence of the endosymbiont within the reproductive tract of female adults. 16S rDNA,ftsZ and groEL gene sequences showed that the endosymbiont of R. similis is distantly related to the known Wolbachia supergroups. Finally, based on our initial success in finding sequences of this endosymbiont by screening an expressed sequence tag (EST) dataset, all nematode ESTs were mined for Wolbachia-like sequences. Although the retained sequences belonged to six different nematode species, R. similis was the only plant-parasitic nematode with traces of Wolbachia. Based on our phylogenetic study and the current literature we designate the endosymbiont of R. similis to a new supergroup (supergroup 1) rather than considering it as a new species. Although its role remains unknown, the endosymbiont was found in all individuals tested, pointing towards an essential function of the bacteria. (C) 2009 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：1045 / 1054

页数：10

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