Subspecies differentiation of Salmonella by PCR-RFLP of the ribosomal operon using universal primers

被引：11

作者：

Shah, SA ^{[1
]}

Romick, TL ^{[1
]}

机构：

[1] HUNT WESSON INC, CTR TECH, FULLERTON, CA 92833 USA

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1997年 / 25卷 / 01期

关键词：

D O I：

10.1046/j.1472-765X.1997.00174.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A polymerase chain reaction (PCR)-based method was developed to aid identification of bacteria to subspecies level. The method used primers that annealed to highly conserved regions of the bacterial rRNA operon, which are proposed to be universal for all bacteria. The resulting PCR products gave unique electrophoretic patterns due to restriction fragment length polymorphisms (RFLP) within the rRNA operon, allowing differentiation to the subspecies level. Six serotypes of Salmonella choleraesuis are presented to demonstrate the specificity of PCR-RFLP patterns for building an identification database. As the database continues to accumulate, the method proves to be specific and rapid for identifying bacteria based on stable genetic characteristics.

引用

页码：54 / 57

页数：4

共 17 条

[1]

ANDREWS WH, 1992, FDA BACTERIOLOGICAL, P51

[2]

Barry T, 1991, PCR Methods Appl, V1, P51

[3] RANDOM AMPLIFIED POLYMORPHIC DNA AND RESTRICTION ENZYME ANALYSIS OF PCR AMPLIFIED RDNA IN TAXONOMY - 2 IDENTIFICATION TECHNIQUES FOR FOOD-BORNE YEASTS [J].