Isolation and Identification of Two Novel SDS-Resistant Secreted Chitinases from Aeromonas schubertii

被引：28

作者：

Liu, Chao-Lin ^{[1
,2
]}

Shen, Chia-Rui ^{[3
]}

Hsu, Fong-Fu ^{[4
]}

Chen, Jeen-Kuan ^{[5
]}

Wu, Pei-Tzu ^{[6
]}

Guo, Shang-Hsin ^{[7
]}

Lee, Wen-Chien ^{[7
]}

Yu, Feng-Wei ^{[8
]}

Mackey, Zachary B. ^{[9
]}

Turk, John ^{[4
]}

Gross, Michael L. ^{[10
]}

机构：

[1] MingChi Univ Technol, Grad Sch Biochem Engn, Taipei, Taiwan

[2] MingChi Univ Technol, Dept Safety Hlth & Environm Engn, Taipei, Taiwan

[3] Chang Gung Univ, Dept Grad Inst Med Biotechnol & Lab Sci, Tao Yuan, Taiwan

[4] Washington Univ, Sch Med, Dept Internal Med, St Louis, MO 63110 USA

[5] Chinese Petr Co, Refine & Manufacture Res Ctr, Dept Environm & Biotechnol, Chiayi, Taiwan

[6] Sun Ten Phytotech Co Ltd, Dept Business Dev, Taipei, Taiwan

[7] Natl Chung Cheng Univ, Dept Chem Engn, Chiayi, Taiwan

[8] Chang Gung Univ, Grad Inst Med Biotechnol, Tao Yuan, Taiwan

[9] Univ Calif San Francisco, Dept Pathol, San Francisco, CA 94143 USA

[10] Washington Univ, Dept Chem, St Louis, MO 63130 USA

来源：

BIOTECHNOLOGY PROGRESS | 2009年 / 25卷 / 01期

关键词：

N-acetyl-D-glucosamine (GlcNAc); Aeromonas schubertii; endochitinase; hexameric chitin oligosaccharide; SDS-resistance; in-gel chitinase assay; HEXA-N-ACETYLCHITOHEXAOSE; SEQUENCE DATABASES; MASS-SPECTROMETRY; MARINE BACTERIUM; CHITOSAN; CLONING; PURIFICATION; EXPRESSION; OLIGOSACCHARIDES; DEGRADATION;

D O I：

10.1002/btpr.100

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two SDS-resistant endochitinases, designated as ASCH153 and ASCH161, were isolated from Aeromonas schubertii in a soil sample from southern Taiwan. MALDI-TOF mass measurement indicates the molecular weights of 53,527 for ASCH153 and 61,202 for ASCHI61. N-terminal and internal amino acid sequences were obtained, and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins. Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide, a compound known to have several potent biomedical functions. ASCH153 and ASCH161 retained, respectively, 65% and 75%, of their chitinase activity in the presence of 5% SDS and 100% of their activity in the presence of 10% beta-mercaptoethanol. These results demonstrate that they are SDS-resistant endochitinases and probably have a rigid structure. 0 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 124-431, 2009

引用

页码：124 / 131

页数：8

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