Enhancement of vitamin E production in sunflower cell cultures

被引：40

作者：

Caretto, S ^{[1
]}

Speth, EB ^{[1
]}

Fachechi, C ^{[1
]}

Gala, R ^{[1
]}

Zacheo, G ^{[1
]}

Giovinazzo, G ^{[1
]}

机构：

[1] CNR, Sez Lecce, Ist Sci Prod Alimentari, I-73100 Lecce, Italy

来源：

PLANT CELL REPORTS | 2004年 / 23卷 / 03期

关键词：

antioxidants; vitamin E; alpha-tocopherol; cell culture; sunflower;

D O I：

10.1007/s00299-004-0799-6

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The most biologically active component of vitamin E, alpha-tocopherol, is synthesized in its most effective stereoisomeric form only by photosynthetic organisms. Using sunflower cell cultures, a suitable in vitro production system of natural alpha-tocopherol was established. The most efficient medium was found to be MS basal medium with naphthaleneacetic acid and 6-benzylaminopurine with the addition of casaminoacids and myo-inositol. Culture feeding experiments using biosynthetic precursors showed that alpha-tocopherol production improved by 30% when homogentisic acid was used. Interestingly, time-course experiments with sunflower suspension cultures showed a possible increase of 78% in alpha-tocopherol production when using cultures of longer subculture intervals. Compared to the starting plant tissue, an overall 100% increase of alpha-tocopherol was reached by these sunflower cell cultures.

引用

页码：174 / 179

页数：6

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