A Primer for Morpholino Use in Zebrafish

被引：299

作者：

Bill, Brent R. ^{[2
,3
]}

Petzold, Andrew M. ^{[1
,4
]}

Clark, Karl J. ^{[1
]}

Schimmenti, Lisa A. ^{[3
,5
,6
]}

Ekker, Stephen C. ^{[1
,2
,3
]}

机构：

[1] Mayo Clin, Dept Biochem & Mol Biol, Rochester, MN 55905 USA

[2] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA

[3] Univ Minnesota, Inst Human Genet, Minneapolis, MN 55455 USA

[4] Univ Minnesota, Dept Comparat & Mol Biosci, St Paul, MN 55108 USA

[5] Univ Minnesota, Dept Pediat Genet & Metab, Minneapolis, MN USA

[6] Univ Minnesota, Dept Ophthalmol, Minneapolis, MN 55455 USA

来源：

ZEBRAFISH | 2009年 / 6卷 / 01期

关键词：

MODEL ORGANISM DATABASE; INFORMATION NETWORK ZFIN; ZINC-FINGER NUCLEASES; ANTISENSE OLIGOMERS; GENE-EXPRESSION; REGENERATION; INHIBITION; EMBRYOS; CELLS; RNA;

D O I：

10.1089/zeb.2008.0555

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Morpholino oligonucleotides are the most common anti-sense "knockdown'' technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.

引用

页码：69 / 77

页数：9

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