Novel genetic tools for studying food-borne Salmonella

被引：18

作者：

Andrews-Polymenis, Helene L. ^{[1
]}

Santiviago, Carlos A. ^{[3
]}

McClelland, Michael ^{[2
]}

机构：

[1] Texas A&M Univ Syst Hlth Sci Ctr, Coll Med, College Stn, TX 77843 USA

[2] Sidney Kimmel Canc Ctr, San Diego, CA 92121 USA

[3] Univ Chile, Fac Med, Programa Microbiol & Micol, Inst Ciencias Biomed ICBM, Santiago 7, Chile

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2009年 / 20卷 / 02期

关键词：

ENTERICA SEROVAR TYPHIMURIUM; SIGNATURE-TAGGED MUTAGENESIS; VIVO EXPRESSION TECHNOLOGY; DIFFERENTIAL FLUORESCENCE INDUCTION; FIELD GEL-ELECTROPHORESIS; BACTERIAL VIRULENCE GENES; COMPLETE GENOME SEQUENCE; SEROTYPE ENTERITIDIS; PROVIDES INSIGHTS; BIOFILM FORMATION;

D O I：

10.1016/j.copbio.2009.02.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nontyphoidal Salmonellae are highly prevalent food-borne pathogens. High-throughput sequencing of Salmonella genomes is expanding our knowledge of the evolution of serovars and epidemic isolates. Genome sequences have also allowed the creation of complete microarrays. Microarrays have improved the throughput of in vivo expression technology (IVET) used to uncover promoters active during infection. In another method, signature tagged mutagenesis (STM), pools of mutants are subjected to selection. Changes in the population are monitored on a microarray, revealing genes under selection. Complete genome sequences permit the construction of pools of targeted in-frame deletions that have improved STM by minimizing the number of clones and the polarity of each mutant. Together, genome sequences and the continuing development of new tools for functional genomics will drive a revolution in the understanding of Salmonellae in many different niches that are critical for food safety.

引用

页码：149 / 157

页数：9

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