Supercritical angle fluorescence (SAF) microscopy

被引：95

作者：

Ruckstuhl, T ^{[1
]}

Verdes, D ^{[1
]}

机构：

[1] Univ Zurich, Inst Phys Chem, CH-8057 Zurich, Switzerland

来源：

OPTICS EXPRESS | 2004年 / 12卷 / 18期

关键词：

D O I：

10.1364/OPEX.12.004246

中图分类号：

O43 [光学];

学科分类号：

070207 [光学]; 0803 [光学工程];

摘要：

We explore a new confocal microscope for the detection of surface-generated fluorescence. The instrument is designed for high resolution imaging as well as for the readout of large biochips. Special feature is the separated collection of two different fluorescence emission modes. One optical path covers the emission into the glass at low surface angles, the other captures high angles, exceeding the critical angle of the water/glass interface. Due to the collection of the supercritical angle fluorescence (SAF) the confocal detection volume is strictly confined to the interface, whereas the low angles collect much deeper from the aqueous analyte solution. Hence the system can deliver information about surface-bound and unbound fraction of fluorescent analyte simultaneously. (C) 2004 Optical Society of America.

引用

页码：4246 / 4254

页数：9

共 15 条

[1]

Axelrod D., 2001, METHODS CELLULAR IMA, P362

[2]

Highly efficient optical detection of surface-generated fluorescence [J].

Enderlein, J ;

Ruckstuhl, T ;

Seeger, S .

APPLIED OPTICS, 1999, 38 (04) :724-732

[3]

Total internal reflection fluorescence correlation spectroscopy for counting molecules at solid/liquid interfaces [J].

Hansen, RL ;

Harris, JM .

ANALYTICAL CHEMISTRY, 1998, 70 (13) :2565-2575

[4]

Real-time detection of nucleotide incorporation during complementary DNA strand synthesis [J].

Krieg, A ;

Laib, S ;

Ruckstuhl, T ;

Seeger, S .

CHEMBIOCHEM, 2003, 4 (07) :589-592

[5]

How to detect weak pairs [J].

Laurence, TA ;

Weiss, S .

SCIENCE, 2003, 299 (5607) :667-668

[6]

Zero-mode waveguides for single-molecule analysis at high concentrations [J].

Levene, MJ ;

Korlach, J ;

Turner, SW ;

Foquet, M ;

Craighead, HG ;

Webb, WW .

SCIENCE, 2003, 299 (5607) :682-686

[7]

Ligand-receptor kinetics measured by total internal reflection with fluorescence correlation spectroscopy [J].

Lieto, AM ;

Cush, RC ;

Thompson, NL .

BIOPHYSICAL JOURNAL, 2003, 85 (05) :3294-3302

[8]

Forbidden light detection from single molecules [J].

Ruckstuhl, T ;

Enderlein, J ;

Jung, S ;

Seeger, S .

ANALYTICAL CHEMISTRY, 2000, 72 (09) :2117-2123

[9]

Attoliter detection volumes by confocal total-internal-reflection fluorescence microscopy [J].

Ruckstuhl, T ;

Seeger, S .

OPTICS LETTERS, 2004, 29 (06) :569-571

[10]

Confocal total-internal-reflection fluorescence microscopy with a high-aperture parabolic mirror lens [J].

Ruckstuhl, T ;

Seeger, S .

APPLIED OPTICS, 2003, 42 (16) :3277-3283

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