Vascular endothelial growth factor receptor-3 in lymphangiogenesis in wound healing

被引:322
作者
Paavonen, K
Puolakkainen, P
Jussila, L
Jahkola, T
Alitalo, K
机构
[1] Univ Helsinki, Haartment Inst, Mol Canc Biol Lab, Helsinki 00014, Finland
[2] Univ Helsinki, Cent Hosp, Dept Surg, Helsinki, Finland
[3] Univ Helsinki, Cent Hosp, Dept Plast Surg, Helsinki, Finland
基金
芬兰科学院;
关键词
D O I
10.1016/S0002-9440(10)65021-3
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Vascular endothelial growth factor receptor-3 (VEGFR-3) is essential for embryonic cardiovascular development, but thereafter becomes confined to the lymphatic endothelium in adult tissues. We have here studied VEGFR-3 expression in experimental wounds of pigs and chronic inflammatory wounds of humans. In healing incisional and punch biopsy wounds made in the dorsal skin of pigs, angiogenic blood vessels, identified by use of the blood vascular endothelial markers vWF: and PAL-E and the basal lamina protein laminin, developed into the granulation tissue stroma from day 4 onward, being most abundant on days 5 and 6 and regressing thereafter. VEGFR-3-positive vessels were observed in the granulation tissue from day 5 onward. These vessels were distinct from the PAL-E/laminin/vWF-positive vessels and fewer in number, and they appeared to sprout from pre-existing VEGFR-3-positive lymphatic vessels at the wound edge. Unlike the blood vessels, very few VEGFR-3-positive lymphatic vessels persisted on day 9 and none on day 14. In chronic wounds such as ulcers and decubitus wounds of the lower extremity of humans, VEGFR-3 was also weakly expressed in the vascular endothelium. Our results suggest that transient lymphangiogenesis occurs in parallel With angiogenesis in healing wounds and that VEGFR-3 becomes up-regulated in blood vessel endothelium in chronic inflammatory wounds.
引用
收藏
页码:1499 / 1504
页数:6
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