The use of resonance energy transfer in high-throughput screening: BRET versus FRET

被引：173

作者：

Boute, N ^{[1
]}

Jockers, R ^{[1
]}

Issad, T ^{[1
]}

机构：

[1] Univ Paris 05, Dept Biol Cellulaire, CNRS UMR 8104, INSERM U567,Inst Cochin, F-75014 Paris, France

来源：

TRENDS IN PHARMACOLOGICAL SCIENCES | 2002年 / 23卷 / 08期

关键词：

D O I：

10.1016/S0165-6147(02)02062-X

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Bioluminescence resonance energy transfer has developed in recent years as a new technique to study protein-protein interactions. Protein partners of interest are tagged with either luciferase or green fluorescent protein (GFP). Non-radiative energy transfer between the excited luciferase and the GFP permits the study of spatial relationships between the two partners. This technique constitutes an important tool for the study of the functional activity of different types of receptors, and can be used in sensitive, homogenous high-throughput screening assays.

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页码：351 / 354

页数：4

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