A conserved capsid protein surface domain of Cucumber mosaic virus is essential for efficient aphid vector transmission

被引:75
作者
Liu, SJ
He, XH
Park, G
Josefsson, C
Perry, KL
机构
[1] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA
[2] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA
关键词
D O I
10.1128/JVI.76.19.9756-9762.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A prominent feature on the surfaces of virions of Cucumber mosaic virus (CMV) is a negatively charged loop structure (the betaH-betaI loop). Six of 8 amino acids in this capsid protein loop are highly conserved among strains of CMV and other cucumoviruses. Five of these amino acids were individually changed to alanine or lysine (an amino acid of opposite charge) to create nine mutants (the D191A, D191K, D192A, D192K, L194A, E195A, E195K, D197A, and D197K mutants). Transcripts of cDNA clones were infectious when they were mechanically inoculated onto tobacco, giving rise to symptoms of a mottle-mosaic typical of the wild-type virus (the D191A, D191K, D192A, E195A, E195K, and D197A mutants), a systemic necrosis (the D192K mutant), or an atypical chlorosis with necrotic flecking (the L194A mutant). The mutants formed virions and accumulated to wild-type levels, but eight of the nine mutants were defective in aphid vector transmission. The aspartate-to-lysine mutation at position 197 interfered with infection; the only recovered progeny (the D197K* mutant) harbored a second-site mutation (denoted by the asterisk) of alanine to glutamate at position 193, a proximal site in the betaH-betaI loop. Since the disruption of charged amino acid residues in the betaH-betaI loop reduces or eliminates vector transmissibility without grossly affecting infectivity or virion formation, we hypothesize that this sequence or structure has been conserved to facilitate aphid vector transmission.
引用
收藏
页码:9756 / 9762
页数:7
相关论文
共 56 条
[1]   MUTATIONAL ANALYSIS OF THE COAT PROTEIN N-TERMINAL AMINO-ACIDS INVOLVED IN POTYVIRUS TRANSMISSION BY APHIDS [J].
ATREYA, PL ;
LOPEZMOYA, JJ ;
CHU, MH ;
ATREYA, CD ;
PIRONE, TP .
JOURNAL OF GENERAL VIROLOGY, 1995, 76 :265-270
[2]   AMINO-ACID SUBSTITUTIONS IN THE COAT PROTEIN RESULT IN LOSS OF INSECT TRANSMISSIBILITY OF A PLANT-VIRUS [J].
ATREYA, PL ;
ATREYA, CD ;
PIRONE, TP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) :7887-7891
[3]   APHID TRANSMISSION OF BEET WESTERN YELLOWS LUTEOVIRUS REQUIRES THE MINOR CAPSID READ-THROUGH PROTEIN P74 [J].
BRAULT, V ;
VANDENHEUVEL, JFJM ;
VERBEEK, M ;
ZIEGLERGRAFF, V ;
REUTENAUER, A ;
HERRBACH, E ;
GARAUD, JC ;
GUILLEY, H ;
RICHARDS, K ;
JONARD, G .
EMBO JOURNAL, 1995, 14 (04) :650-659
[4]   The multifunctional capsid proteins of plant RNA viruses [J].
Callaway, A ;
Giesman-Cookmeyer, D ;
Gillock, ET ;
Sit, TL ;
Lommel, SA .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 2001, 39 :419-+
[5]   CUCUMOVIRUS TRANSMISSION BY THE APHID MYZUS-PERSICAE IS DETERMINED SOLELY BY THE VIRAL COAT PROTEIN [J].
CHEN, B ;
FRANCKI, RIB .
JOURNAL OF GENERAL VIROLOGY, 1990, 71 :939-944
[6]  
David P. A., 1988, Information Economics and Policy, V3, P165, DOI 10.1016/0167-6245(88)90024-8
[7]   Expression and suppression of circulative aphid transmission in pea enation mosaic virus [J].
Demler, SA ;
RuckerFeeney, DG ;
Skaf, JS ;
deZoeten, GA .
JOURNAL OF GENERAL VIROLOGY, 1997, 78 :511-523
[8]   An interspecies hybrid RNA virus is significantly more virulent than either parental virus [J].
Ding, SW ;
Shi, BJ ;
Li, WX ;
Symons, RH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (15) :7470-7474
[9]   A NOVEL NATURALLY-OCCURRING HYBRID GENE ENCODED BY A PLANT RNA VIRUS FACILITATES LONG-DISTANCE VIRUS MOVEMENT [J].
DING, SW ;
LI, WX ;
SYMONS, RH .
EMBO JOURNAL, 1995, 14 (23) :5762-5772
[10]  
Francki RIB., 1979, CMI AAB DESCRIPTIONS