The chemokine receptor CXCR2 controls positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration

被引:295
作者
Tsai, HH
Frost, E
To, V
Robinson, S
ffrench-Constant, C
Geertman, R
Ransohoff, RM
Miller, RH [1 ]
机构
[1] Case Western Reserve Univ, Sch Med, Dept Neurosci, Cleveland, OH 44106 USA
[2] Uniformed Serv Univ Hlth Sci, Dept Anat & Cell Biol, Bethesda, MD 20814 USA
[3] Univ Hosp Cleveland, Res Inst, Dept Neurosurg, Cleveland, OH 44106 USA
[4] Cambridge Ctr Brain Repair, Cambridge CB2 2PY, England
[5] Cleveland Clin Fdn, Lerner Res Inst, Dept Neurol & Neurosci, Cleveland, OH 44195 USA
关键词
D O I
10.1016/S0092-8674(02)00838-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Spinal cord oligodendrocytes originate in the ventricular zone and subsequently migrate to white matter, stop, proliferate, and differentiate. Here we demonstrate a role for the chemokine CXCL1 and its receptor CXCR2 in patterning the developing spinal cord. Signaling through CXCR2, CXCL1 inhibited oligodendrocyte precursor migration. The migrational arrest was rapid, reversible, concentration dependent, and reflected enhanced cell/substrate interactions. White matter expression of CXCL1 was temporo-spatially regulated. Developing CXCR2 null spinal cords contained reduced oligodendrocytes, abnormally concentrated at the periphery. In slice preparations, CXCL1 inhibited embryonic oligodendrocyte precursor migration, and widespread dispersal of postnatal precursors occurred in the absence of CXCR2 signaling. These data suggest that population of presumptive white matter by oligodendrocyte precursors is dependent on localized expression of CXCL1.
引用
收藏
页码:373 / 383
页数:11
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