ERK activation upon hypoxia: involvement in HIF-1 activation

被引：327

作者：

Minet, E ^{[1
]}

Arnould, T ^{[1
]}

Michel, G ^{[1
]}

Roland, I ^{[1
]}

Mottet, D ^{[1
]}

Raes, M ^{[1
]}

Remacle, J ^{[1
]}

Michiels, C ^{[1
]}

机构：

[1] Fac Univ Notre Dame Paix, Lab Biochim & Biol Cellulaire, B-5000 Namur, Belgium

来源：

FEBS LETTERS | 2000年 / 468卷 / 01期

关键词：

hypoxia; hypoxia-inducible factor-1 (HIF-1); extracellular regulated kinase 1 and 2 (ERK);

D O I：

10.1016/S0014-5793(00)01181-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia, The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia, Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIT-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1 alpha is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia, (C) 2000 Federation of European Biochemical Societies.

引用

页码：53 / 58

页数：6

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