Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit

被引:341
作者
Garay-Arroyo, A
Colmenero-Flores, JM
Garciarrubio, A
Covarrubias, AA
机构
[1] Univ Nacl Autonoma Mexico, Dept Plant Mol Biol, Inst Biotechnol, Cuernavaca 62250, Morelos, Mexico
[2] Univ Nacl Autonoma Mexico, Dept Biostruct & Reconocimiento Mol, Inst Biotechnol, Cuernavaca 62250, Morelos, Mexico
关键词
D O I
10.1074/jbc.275.8.5668
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The late embryogenesis abundant (LEA) proteins are plant proteins that are synthesized at the onset of desiccation in maturing seeds and in vegetative organs exposed to water deficit. Here, we show that most LEA proteins are comprised in a more widespread group, which we call "hydrophilins," The defining characteristics of hydrophilins are high glycine content (>6%) and a high hydrophilicity index (>1.0). By data base searching, we show that this criterion selectively differentiates most known LEA proteins as well as additional proteins from different taxons. We found that within the genomes of Escherichia coli and Saccharomyces cerevisiae, only 5 and 12 proteins, respectively, meet our criterion. Despite their deceivingly loose definition, hydrophilins usually represent <0.2% of the proteins of a genome. Additionally, we demonstrate that the criterion that defines hydrophilins seems to be an excellent predictor of responsiveness to hyperosmosis since most of the genes encoding these proteins in E. coli and S. cerevisiae are induced by osmotic stress. Evidence for the participation of one of the E. coli hydrophilins in the adaptive response to hyperosmotic conditions is presented. Apparently, hydrophilins represent analogous adaptations to a common problem in such diverse taxons as prokaryotes and eukaryotes.
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页码:5668 / 5674
页数:7
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