On-line sample preconcentration by sweeping with dodecyltrimethylammonium bromide in capillary zone electrophoresis

被引:21
作者
Gong, Maojun
Wehmeyer, Kenneth R.
Limbach, Patrick A.
Heineman, William R.
机构
[1] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA
[2] Hlth Care Res Ctr, Mason, OH 45040 USA
[3] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA
关键词
sweeping; capillary zone electrophoresis; sample preconcentration; thrombin aptamers; dodecyltrimethylammonium bromide;
D O I
10.1016/j.chroma.2006.05.058
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
On-line sample preconcentration of oligonucleotides with a new sweeping carrier was developed by using dodecyltrimethylammonium bromide (DTAB) below the critical micelle concentration (CMC). The sweeping results with DTAB below and above the CMC were compared. The use of DTAB below the CMC benefits the preconcentration of the oligonucleotides, while the use of DTAB above the CMC is good for hydrophobic small molecules. The factors affecting the sweeping results were optimized and this method was evaluated by constructing calibration curves for thrombin aptamers. The sweeping scheme produced a 112-fold sensitivity enhancement for the oligonucleotides relative to that run in a running buffer without DTAB. The sweeping method developed here can be a good reinforcement of the preconcentration scheme by sweeping when less-hydrophobic analytes or large negatively-charged molecules need to be preconcentrated. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:263 / 269
页数:7
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