Respiratory viruses in bronchoalveolar lavage: a hospital-based cohort study in adults

被引:93
作者
Garbino, J. [1 ]
Soccal, P. M. [2 ,3 ]
Aubert, J-D [4 ]
Rochat, T. [2 ]
Meylan, P. [5 ,6 ]
Thomas, Y. [1 ]
Tapparel, C. [1 ]
Bridevaux, P-O [2 ]
Kaiser, L. [1 ]
机构
[1] Univ Hosp Geneva, Div Infect Dis, Cent Lab Virol, CH-1211 Geneva 14, Switzerland
[2] Univ Hosp Geneva, Div Pulm Med, CH-1211 Geneva, Switzerland
[3] Univ Hosp Geneva, Clin Thorac Surg, CH-1211 Geneva 14, Switzerland
[4] Univ Lausanne Hosp, Div Pulm Med, Lausanne, Switzerland
[5] Univ Lausanne Hosp, Inst Microbiol, Lausanne, Switzerland
[6] Univ Lausanne Hosp, Div Infect Dis, Lausanne, Switzerland
关键词
HUMAN BOCAVIRUS; CORONAVIRUS INFECTIONS; EPIDEMIOLOGIC PROFILE; HUMAN RHINOVIRUS; CLINICAL IMPACT; LOWER AIRWAYS; EPITHELIUM; INFANTS; DISEASE; SAMPLES;
D O I
10.1136/thx.2008.105155
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: The epidemiology of respiratory viruses and their potential clinical impact when recovered in lower respiratory specimens has not been established in the hospital setting. A study was performed to investigate the association between positive viral detection and respiratory infection in an at-risk population. Methods: 299 adult patients who underwent bronchoalveolar lavage (BAL) procedures were enrolled in a hospital-based prospective cohort study. Descriptive epidemiology is presented of 17 different respiratory viruses detected by reverse transcription-polymerase chain reaction assays in BAL fluid specimens. Multivariate analysis was conducted to identify the clinical characteristics independently associated with the presence of virus. Results: Of 522 BAL fluid specimens analysed, 81% were collected in adult transplant recipients or other immuno-compromised patients. Overall, PCR assays identified viral nucleic acid in 91 BAL fluid samples (17.4%). Similar rates of virus-positive BAL fluid were found in the different subpopulations studied (p=0.113). Coronaviruses were the most frequent (32.3%), followed by rhinovirus (22.6%), parainfluenza (19.5%), influenza (9.7%), respiratory synctial virus (8.6%), human metapneumovirus (4.2%) and bocavirus (3.1%). Multivariate analysis using mixed models showed that respiratory viral infections were associated with a lack of antibiotic treatment response (OR 2.2, 95% CI 1.2 to 4.1) and the absence of radiological infiltrate (OR 0.3, 95% CI 0.2 to 0.8). In lung transplant recipients in whom a respiratory infection was suspected, the respiratory viral detection rate was 24.4% compared with 13.8% overall in other patients (p=0.02). Conclusions: In this cohort of hospitalised adults, respiratory viruses detected in BAL fluid specimens were associated with respiratory symptoms, absence of radiological infiltrates and a poor response to antibiotic therapy.
引用
收藏
页码:399 / 404
页数:6
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