Bovine adrenal endothelial cells express nucleoside transporters nonregulated by protein kinases A and C

被引:10
作者
Sen, RP
Sobrevia, L
Delicado, EG
Yudilevich, D
MirasPortugal, MT
机构
[1] UNIV COMPLUTENSE MADRID, FAC VET, DEPT BIOQUIM, MADRID 28040, SPAIN
[2] UNIV CHILE, FAC MED, DEPT PHYSIOL & BIOPHYS, SANTIAGO, CHILE
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 271卷 / 02期
关键词
adenosine transport; adrenal medulla; adenosine; 3'; 5'-cyclic monophosphate; nitrobenzylthioinosine; nucleoside transport;
D O I
10.1152/ajpcell.1996.271.2.C504
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The present investigation characterizes the nucleoside transporters in bovine adrenomedullary endothelial cells and their possible regulation by the action of protein kinases A and C to establish comparisons with the nucleoside transport, system in chromaffin cells. The nucleoside transport proved to be a nitrobenzylthioinosine (NBTI)-sensitive facilitated-diffusion system with high affinity for adenosine. These endothelial cells had a high density of nucleoside transporters (660,000 +/- 130,000 transporters/cell), measured by NBTI binding, and the efficiency was close to 2 adenosine molecules internalized . transporter(-1). s(-1). The stimulation of the cells with bradykinin and P-1,P-4-di(adenosine-5')tetraphosphate, which raise the intra cellular Ca2+ concentration, did not modulate the adenosine transport. When the cells were stimulated with signals coupled to adenosine 3',5'-cyclic monophosphate intracellular production, such as norepinephrine and isoproterenol, the adenosine transport was not modified. Furthermore, the treatment of the cells with direct activators of both protein kinases A and C had no effect on adenosine transport, in contrast to that reported in chromaffin cells.
引用
收藏
页码:C504 / C510
页数:7
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